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P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.

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Title: P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.
Authors: Woodell, J E1, Laberge, M1, Langan III, E M2, Hilderman, R H3
Source: Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing). Jan2003, Vol. 217 Issue 1, p21-26. 6p.
Subjects: Endothelium, Cells, Angioplasty, DNA synthesis, Nitric oxide
Abstract: Rapid re-endothelialization following balloon angioplasty can reduce restenosis by inhibiting smooth muscle cell migration and proliferation. However, formation of a neointima layer following angioplasty can be inhibited due to endothelial cell dysfunction and denudation. In a companion paper, it has been illustrated that mechanical loading causes a decrease in DNA synthesis in bovine aortic endothelial cells (BAECs) thus rendering them dysfunctional. The purpose of this study was to overcome BAEC dysfunction by incubation with pharmacological agents to increase DNA synthesis. Previous studies demonstrated that the adenosine dinucleotides Ap[sub 4]A and Ap[sub 2]A induced nitric oxide (NO) production from BAEC while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A did not. This paper demonstrates that Ap[sub 4]A and Ap[sub 2]A induce a 1.46- and 1.16-fold increase in DNA synthesis in mechanically stressed BAECs respectively, while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A do not. Additionally, NOC-18, a slow NO release NO donor, significantly increases DNA synthesis in mechanically stressed BAECs without affecting unloaded cells. These results are consistent with NO inducing DNA synthesis in mechanically stressed BAECs. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing) is the property of Professional Engineering Publishing and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 9120920
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PubType: Academic Journal
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  Data: P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.
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  Data: <searchLink fieldCode="DE" term="%22Endothelium%22">Endothelium</searchLink><br /><searchLink fieldCode="DE" term="%22Cells%22">Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Angioplasty%22">Angioplasty</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+synthesis%22">DNA synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Nitric+oxide%22">Nitric oxide</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Rapid re-endothelialization following balloon angioplasty can reduce restenosis by inhibiting smooth muscle cell migration and proliferation. However, formation of a neointima layer following angioplasty can be inhibited due to endothelial cell dysfunction and denudation. In a companion paper, it has been illustrated that mechanical loading causes a decrease in DNA synthesis in bovine aortic endothelial cells (BAECs) thus rendering them dysfunctional. The purpose of this study was to overcome BAEC dysfunction by incubation with pharmacological agents to increase DNA synthesis. Previous studies demonstrated that the adenosine dinucleotides Ap[sub 4]A and Ap[sub 2]A induced nitric oxide (NO) production from BAEC while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A did not. This paper demonstrates that Ap[sub 4]A and Ap[sub 2]A induce a 1.46- and 1.16-fold increase in DNA synthesis in mechanically stressed BAECs respectively, while Ap[sub 3]A, Ap[sub 5]A and Ap[sub 6]A do not. Additionally, NOC-18, a slow NO release NO donor, significantly increases DNA synthesis in mechanically stressed BAECs without affecting unloaded cells. These results are consistent with NO inducing DNA synthesis in mechanically stressed BAECs. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing) is the property of Professional Engineering Publishing and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1243/095441103762597700
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        Text: English
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      – SubjectFull: Endothelium
        Type: general
      – SubjectFull: Cells
        Type: general
      – SubjectFull: Angioplasty
        Type: general
      – SubjectFull: DNA synthesis
        Type: general
      – SubjectFull: Nitric oxide
        Type: general
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      – TitleFull: P[sup 1],P[sup 4]-diadenosine 5'-tetraphosphate induced DNA synthesis in mechanically injured cultured endothelial cells.
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            NameFull: Laberge, M
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            NameFull: Langan III, E M
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              Text: Jan2003
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              Y: 2003
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            – TitleFull: Proceedings of the Institution of Mechanical Engineers -- Part H -- Journal of Engineering in Medicine (Professional Engineering Publishing)
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