Bibliographic Details
| Title: |
Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment. |
| Authors: |
Millard, Julie T.1 jtmillar@colby.edu, Chuang, Edward1, Lucas, James S.1, Nagy, Erzsebet E.1, Davis, Griffin T.1 |
| Source: |
Journal of Chemical Education. Nov2013, Vol. 90 Issue 11, p1518-1521. 4p. |
| Subjects: |
Horse research, Biochemistry experiments, Biochemistry education, Restriction fragment length polymorphisms, Polymerase chain reaction |
| Abstract: |
A simple and robust biochemistry laboratory experiment is described that uses restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products to verify the identity of a potentially valuable horse. During the first laboratory period, students purify DNA from equine samples and amplify two loci of mitochondrial DNA. During the second laboratory period, students digest PCR products with restriction enzymes and analyze the fragment sizes through agarose gel electrophoresis. An optional step of validating DNA extracts through real-time PCR can expand the experiment to three weeks. This experiment, which has an engaging and versatile scenario, provides students with exposure to key principles and techniques of molecular biology, bioinformatics, and evolution in a forensic context. [ABSTRACT FROM AUTHOR] |
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| Database: |
Engineering Source |
