Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment.
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| Title: | Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment. |
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| Authors: | Millard, Julie T.1 jtmillar@colby.edu, Chuang, Edward1, Lucas, James S.1, Nagy, Erzsebet E.1, Davis, Griffin T.1 |
| Source: | Journal of Chemical Education. Nov2013, Vol. 90 Issue 11, p1518-1521. 4p. |
| Subjects: | Horse research, Biochemistry experiments, Biochemistry education, Restriction fragment length polymorphisms, Polymerase chain reaction |
| Abstract: | A simple and robust biochemistry laboratory experiment is described that uses restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products to verify the identity of a potentially valuable horse. During the first laboratory period, students purify DNA from equine samples and amplify two loci of mitochondrial DNA. During the second laboratory period, students digest PCR products with restriction enzymes and analyze the fragment sizes through agarose gel electrophoresis. An optional step of validating DNA extracts through real-time PCR can expand the experiment to three weeks. This experiment, which has an engaging and versatile scenario, provides students with exposure to key principles and techniques of molecular biology, bioinformatics, and evolution in a forensic context. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Chemical Education is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 92897644 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Millard%2C+Julie+T%2E%22">Millard, Julie T.</searchLink><relatesTo>1</relatesTo><i> jtmillar@colby.edu</i><br /><searchLink fieldCode="AR" term="%22Chuang%2C+Edward%22">Chuang, Edward</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lucas%2C+James+S%2E%22">Lucas, James S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nagy%2C+Erzsebet+E%2E%22">Nagy, Erzsebet E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Davis%2C+Griffin+T%2E%22">Davis, Griffin T.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Chemical+Education%22">Journal of Chemical Education</searchLink>. Nov2013, Vol. 90 Issue 11, p1518-1521. 4p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Horse+research%22">Horse research</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry+experiments%22">Biochemistry experiments</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry+education%22">Biochemistry education</searchLink><br /><searchLink fieldCode="DE" term="%22Restriction+fragment+length+polymorphisms%22">Restriction fragment length polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: A simple and robust biochemistry laboratory experiment is described that uses restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products to verify the identity of a potentially valuable horse. During the first laboratory period, students purify DNA from equine samples and amplify two loci of mitochondrial DNA. During the second laboratory period, students digest PCR products with restriction enzymes and analyze the fragment sizes through agarose gel electrophoresis. An optional step of validating DNA extracts through real-time PCR can expand the experiment to three weeks. This experiment, which has an engaging and versatile scenario, provides students with exposure to key principles and techniques of molecular biology, bioinformatics, and evolution in a forensic context. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Chemical Education is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1021/ed300740r Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 1518 Subjects: – SubjectFull: Horse research Type: general – SubjectFull: Biochemistry experiments Type: general – SubjectFull: Biochemistry education Type: general – SubjectFull: Restriction fragment length polymorphisms Type: general – SubjectFull: Polymerase chain reaction Type: general Titles: – TitleFull: Case-Study Investigation of Equine Maternity via PCR-RFLP: A Biochemistry Laboratory Experiment. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Millard, Julie T. – PersonEntity: Name: NameFull: Chuang, Edward – PersonEntity: Name: NameFull: Lucas, James S. – PersonEntity: Name: NameFull: Nagy, Erzsebet E. – PersonEntity: Name: NameFull: Davis, Griffin T. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2013 Type: published Y: 2013 Identifiers: – Type: issn-print Value: 00219584 Numbering: – Type: volume Value: 90 – Type: issue Value: 11 Titles: – TitleFull: Journal of Chemical Education Type: main |
| ResultId | 1 |
