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Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy.

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Title: Microscale receiver operating characteristic analysis of micrometastasis recognition using activatable fluorescent probes indicates leukocyte imaging as a critical factor to enhance accuracy.
Authors: Spring, Bryan Q.1, Palanisami, Akilan1, Hasan, Tayyaba1,2,3 thasan@mgh.harvard.edu
Source: Journal of Biomedical Optics. Jun2014, Vol. 19 Issue 6, p1-5. 5p.
Subjects: Micrometastasis, Leukocytes, Accuracy, Tissue wounds, Biopsy
Abstract: Molecular-targeted probes are emerging with applications for optical biopsy of cancer. An underex-plored potential clinical use of these probes is to monitor residual cancer micrometastases that escape cyto-reductive surgery and chemotherapy. Here, we show that leukocytes, or white blood cells, residing in nontumor tissues--as well as those infiltrating micrometastatic lesions--uptake cancer cell-targeted, activatable immuno-conjugates nonspecifically, which limits the accuracy and resolution of micrometastasis recognition using these probes. Receiver operating characteristic analysis of freshly excised tissues from a mouse model of peritoneal carcinomatosis suggests that dual-color imaging, adding an immunostain for leukocytes, offers promise for enabling accurate recognition of single cancer cells. Our results indicate that leukocyte identification improves micrometastasis recognition sensitivity and specificity from 92 to 93%--for multicellular metastases >20 to 30 μm in size--to 98 to 99.9% for resolving metastases as small as a single cell. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Molecular-targeted probes are emerging with applications for optical biopsy of cancer. An underex-plored potential clinical use of these probes is to monitor residual cancer micrometastases that escape cyto-reductive surgery and chemotherapy. Here, we show that leukocytes, or white blood cells, residing in nontumor tissues--as well as those infiltrating micrometastatic lesions--uptake cancer cell-targeted, activatable immuno-conjugates nonspecifically, which limits the accuracy and resolution of micrometastasis recognition using these probes. Receiver operating characteristic analysis of freshly excised tissues from a mouse model of peritoneal carcinomatosis suggests that dual-color imaging, adding an immunostain for leukocytes, offers promise for enabling accurate recognition of single cancer cells. Our results indicate that leukocyte identification improves micrometastasis recognition sensitivity and specificity from 92 to 93%--for multicellular metastases >20 to 30 μm in size--to 98 to 99.9% for resolving metastases as small as a single cell. [ABSTRACT FROM AUTHOR]
ISSN:10833668
DOI:10.1117/1.JBO.19.6.066006