M13 bacteriophage production for large-scale applications.

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Bibliographic Details
Title:	M13 bacteriophage production for large-scale applications.
Authors:	Warner, Christopher¹ christopher.m.warner@usace.army.mil, Barker, Natalie¹, Lee, Seung-Wuk, Perkins, Edward¹
Source:	Bioprocess & Biosystems Engineering. Oct2014, Vol. 37 Issue 10, p2067-2072. 6p.
Subjects:	Bacteriophages, Microbial biotechnology, Temperature effect, Bacterial growth, Mathematical optimization
Abstract:	Bacteriophage materials have the potential to revolutionize medicine, energy production and storage, agriculture, solar cells, optics and many other fields. To fulfill these needs, this study examined critical process parameters during phage propagation to increase phage production capability. A representative scale-down system was created in tube spin reactors to allow parallel experimentation with single- and multi-variable analysis. Temperature, harvest time, media composition, feed regime, bacteriophage, and bacteria concentration were analyzed in the scale-down system. Temperature, media composition, and feeding regimens were found to affect phage production more than other factors. Temperature affected bacterial growth and phage production inversely. Multi-variate analysis identified an optimal parameter space which provided a significant improvement over the base line method. This method should be useful in scaled production of bacteriophage for biotechnology. [ABSTRACT FROM AUTHOR]
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Database:	Engineering Source

Description
Abstract:	Bacteriophage materials have the potential to revolutionize medicine, energy production and storage, agriculture, solar cells, optics and many other fields. To fulfill these needs, this study examined critical process parameters during phage propagation to increase phage production capability. A representative scale-down system was created in tube spin reactors to allow parallel experimentation with single- and multi-variable analysis. Temperature, harvest time, media composition, feed regime, bacteriophage, and bacteria concentration were analyzed in the scale-down system. Temperature, media composition, and feeding regimens were found to affect phage production more than other factors. Temperature affected bacterial growth and phage production inversely. Multi-variate analysis identified an optimal parameter space which provided a significant improvement over the base line method. This method should be useful in scaled production of bacteriophage for biotechnology. [ABSTRACT FROM AUTHOR]
ISSN:	16157591
DOI:	10.1007/s00449-014-1184-7