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High-throughput intracellular pteridinic profiling by liquid chromatography–quadrupole time-of-flight mass spectrometry.

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Title: High-throughput intracellular pteridinic profiling by liquid chromatography–quadrupole time-of-flight mass spectrometry.
Authors: Burton, Casey1, Weng, Rui2, Yang, Li2, Bai, Yu2 yu.bai@pku.edu.cn, Liu, Huwei2, Ma, Yinfa1 yinfa@mst.edu
Source: Analytica Chimica Acta. Jan2015, Vol. 853, p442-450. 9p.
Subjects: Pteridines, Biomarkers, Body fluid analysis, Extraction (Chemistry), Time-of-flight mass spectrometry, Liquid chromatography
Abstract: Pteridines are a diverse family of endogenous metabolites that may serve as useful diagnostic biomarkers for disease. While many preparative and analytical techniques have been described for analysis of selected pteridines in biological fluids, broad intracellular pteridine detection remains a significant analytical challenge. In this study, a novel, specific and sensitive extraction and high performance liquid chromatography–quadrupole time-of-flight mass spectrometry (HPLC–QTOF MS) method was developed to simultaneously quantify seven intracellular pteridines and monitor 18 additional, naturally-occurring intracellular pteridines. The newly developed method was validated through evaluation of spiked recoveries (84.5–109.4%), reproducibility (2.1–5.4% RSD), method detection limits (0.1–3.0 μg L −1 ) and limits of quantitation (0.1–1 μg L −1 ), and finally application to non-small cell lung cancer A549 cells. Twenty-three pteridine derivatives were successfully detected from cell lysates with an average RSD of 12% among culture replicates. Quantified intracellular pteridine levels ranged from 1 to 1000 nM in good agreement with previous studies. Finally, this technique may be applied to cellular studies to generate new biological hypotheses concerning pteridine physiological and pathological functions as well as to discovery new pteridine-based biomarkers. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Pteridines are a diverse family of endogenous metabolites that may serve as useful diagnostic biomarkers for disease. While many preparative and analytical techniques have been described for analysis of selected pteridines in biological fluids, broad intracellular pteridine detection remains a significant analytical challenge. In this study, a novel, specific and sensitive extraction and high performance liquid chromatography–quadrupole time-of-flight mass spectrometry (HPLC–QTOF MS) method was developed to simultaneously quantify seven intracellular pteridines and monitor 18 additional, naturally-occurring intracellular pteridines. The newly developed method was validated through evaluation of spiked recoveries (84.5–109.4%), reproducibility (2.1–5.4% RSD), method detection limits (0.1–3.0 μg L −1 ) and limits of quantitation (0.1–1 μg L −1 ), and finally application to non-small cell lung cancer A549 cells. Twenty-three pteridine derivatives were successfully detected from cell lysates with an average RSD of 12% among culture replicates. Quantified intracellular pteridine levels ranged from 1 to 1000 nM in good agreement with previous studies. Finally, this technique may be applied to cellular studies to generate new biological hypotheses concerning pteridine physiological and pathological functions as well as to discovery new pteridine-based biomarkers. [ABSTRACT FROM AUTHOR]
ISSN:00032670
DOI:10.1016/j.aca.2014.10.044