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Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.

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Title: Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.
Authors: Dwenger, Kaitlyn1 kdwenger@purdue.edu, do Nascimento, Naila Cannes1, Bailey, Taylor W.1, Xie, Jun2, Cox, Abigail3, Schaser, Allison J.1, Sivasankara, M. Preeti1
Source: Journal of Speech, Language & Hearing Research. Jul2026, Vol. 69 Issue 7, p2940-2960. 21p.
Subject Terms: *Data analysis, *Comparative studies, Laryngeal physiology, Biological models, Statistical power analysis, Statistical models, Drinking (Physiology), Animal communication, Research funding, T-test (Statistics), Ultrasonics, Body weight, Sample size (Statistics), Enzyme-linked immunosorbent assay, Two-way analysis of variance, Estrogen, Reverse transcriptase polymerase chain reaction, Descriptive statistics, Chi-squared test, Estradiol, Rats, Serum, Gene expression, Reward (Psychology), Animal experimentation, Animal behavior, Statistics, Water, Data analysis software, Anthropometry, Biomarkers, Dehydration, Uterus
Abstract: Purpose: Hormonal and hydration states are known to individually influence laryngeal physiology, yet their distinct and combined effects on vocal function remain poorly understood. This study investigated the interaction of reduced circulating estrogen, estradiol replacement, and mild systemic dehydration (DEHY) on ultrasonic vocalization (USV) in adult female rats. Method: Forty-eight Long–Evans rats were assigned to six groups (n = 8 per group) that differed in hormonal state (control, ovariectomized + estradiol pellet [OVX + Estradiol], OVX + placebo pellet) and hydration status (euhydration [EUHY], DEHY). USVs were recorded during a food-reward elicitation paradigm before hormonal (pellet) replacement, after hormonal replacement, and after 5 days of EUHY or DEHY. Dependent measures included USV number, duration, frequency range, and maximum power. Physiological measures (body weight, water intake, kidney renin expression, uterine weight) supported experimental manipulations. Results: OVX animals produced shorter duration and lower power USVs compared with controls, although total number and frequency range were largely preserved. Even after hormonal replacement, OVX + Estradiol animals emitted fewer USVs with shorter duration, narrower frequency range, and reduced power as compared with control animals. DEHY narrowed USV frequency range across hormonal groups and interacted with hormonal status to modulate USV number and duration. Conclusions: Both circulating estrogen and systemic hydration status selectively influenced USV profiles. Although statistically significant, USV changes were generally of small magnitude, warranting caution in interpretation. These findings highlight the nuanced and context-dependent nature of endocrine and hydration influences on vocalizations and establish a preclinical framework for investigating hormone–hydration interactions in laryngeal function. [ABSTRACT FROM AUTHOR]
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Database: Education Research Complete
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Abstract:Purpose: Hormonal and hydration states are known to individually influence laryngeal physiology, yet their distinct and combined effects on vocal function remain poorly understood. This study investigated the interaction of reduced circulating estrogen, estradiol replacement, and mild systemic dehydration (DEHY) on ultrasonic vocalization (USV) in adult female rats. Method: Forty-eight Long–Evans rats were assigned to six groups (n = 8 per group) that differed in hormonal state (control, ovariectomized + estradiol pellet [OVX + Estradiol], OVX + placebo pellet) and hydration status (euhydration [EUHY], DEHY). USVs were recorded during a food-reward elicitation paradigm before hormonal (pellet) replacement, after hormonal replacement, and after 5 days of EUHY or DEHY. Dependent measures included USV number, duration, frequency range, and maximum power. Physiological measures (body weight, water intake, kidney renin expression, uterine weight) supported experimental manipulations. Results: OVX animals produced shorter duration and lower power USVs compared with controls, although total number and frequency range were largely preserved. Even after hormonal replacement, OVX + Estradiol animals emitted fewer USVs with shorter duration, narrower frequency range, and reduced power as compared with control animals. DEHY narrowed USV frequency range across hormonal groups and interacted with hormonal status to modulate USV number and duration. Conclusions: Both circulating estrogen and systemic hydration status selectively influenced USV profiles. Although statistically significant, USV changes were generally of small magnitude, warranting caution in interpretation. These findings highlight the nuanced and context-dependent nature of endocrine and hydration influences on vocalizations and establish a preclinical framework for investigating hormone–hydration interactions in laryngeal function. [ABSTRACT FROM AUTHOR]
ISSN:10924388
DOI:10.1044/2026_JSLHR-25-00912