View in EDS PDF Full Text

Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.

Saved in:
Bibliographic Details
Title: Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.
Authors: Dwenger, Kaitlyn1 kdwenger@purdue.edu, do Nascimento, Naila Cannes1, Bailey, Taylor W.1, Xie, Jun2, Cox, Abigail3, Schaser, Allison J.1, Sivasankara, M. Preeti1
Source: Journal of Speech, Language & Hearing Research. Jul2026, Vol. 69 Issue 7, p2940-2960. 21p.
Subject Terms: *Data analysis, *Comparative studies, Laryngeal physiology, Biological models, Statistical power analysis, Statistical models, Drinking (Physiology), Animal communication, Research funding, T-test (Statistics), Ultrasonics, Body weight, Sample size (Statistics), Enzyme-linked immunosorbent assay, Two-way analysis of variance, Estrogen, Reverse transcriptase polymerase chain reaction, Descriptive statistics, Chi-squared test, Estradiol, Rats, Serum, Gene expression, Reward (Psychology), Animal experimentation, Animal behavior, Statistics, Water, Data analysis software, Anthropometry, Biomarkers, Dehydration, Uterus
Abstract: Purpose: Hormonal and hydration states are known to individually influence laryngeal physiology, yet their distinct and combined effects on vocal function remain poorly understood. This study investigated the interaction of reduced circulating estrogen, estradiol replacement, and mild systemic dehydration (DEHY) on ultrasonic vocalization (USV) in adult female rats. Method: Forty-eight Long–Evans rats were assigned to six groups (n = 8 per group) that differed in hormonal state (control, ovariectomized + estradiol pellet [OVX + Estradiol], OVX + placebo pellet) and hydration status (euhydration [EUHY], DEHY). USVs were recorded during a food-reward elicitation paradigm before hormonal (pellet) replacement, after hormonal replacement, and after 5 days of EUHY or DEHY. Dependent measures included USV number, duration, frequency range, and maximum power. Physiological measures (body weight, water intake, kidney renin expression, uterine weight) supported experimental manipulations. Results: OVX animals produced shorter duration and lower power USVs compared with controls, although total number and frequency range were largely preserved. Even after hormonal replacement, OVX + Estradiol animals emitted fewer USVs with shorter duration, narrower frequency range, and reduced power as compared with control animals. DEHY narrowed USV frequency range across hormonal groups and interacted with hormonal status to modulate USV number and duration. Conclusions: Both circulating estrogen and systemic hydration status selectively influenced USV profiles. Although statistically significant, USV changes were generally of small magnitude, warranting caution in interpretation. These findings highlight the nuanced and context-dependent nature of endocrine and hydration influences on vocalizations and establish a preclinical framework for investigating hormone–hydration interactions in laryngeal function. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Speech, Language & Hearing Research is the property of American Speech-Language-Hearing Association and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Education Research Complete
FullText Links:
  – Type: pdflink
Text:
  Availability: 0
Header DbId: ehh
DbLabel: Education Research Complete
An: 195295658
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Dwenger%2C+Kaitlyn%22">Dwenger, Kaitlyn</searchLink><relatesTo>1</relatesTo><i> kdwenger@purdue.edu</i><br /><searchLink fieldCode="AR" term="%22do+Nascimento%2C+Naila+Cannes%22">do Nascimento, Naila Cannes</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bailey%2C+Taylor+W%2E%22">Bailey, Taylor W.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Xie%2C+Jun%22">Xie, Jun</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cox%2C+Abigail%22">Cox, Abigail</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Schaser%2C+Allison+J%2E%22">Schaser, Allison J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sivasankara%2C+M%2E+Preeti%22">Sivasankara, M. Preeti</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Speech%2C+Language+%26+Hearing+Research%22">Journal of Speech, Language & Hearing Research</searchLink>. Jul2026, Vol. 69 Issue 7, p2940-2960. 21p.
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: *<searchLink fieldCode="DE" term="%22Data+analysis%22">Data analysis</searchLink><br />*<searchLink fieldCode="DE" term="%22Comparative+studies%22">Comparative studies</searchLink><br /><searchLink fieldCode="DE" term="%22Laryngeal+physiology%22">Laryngeal physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+models%22">Biological models</searchLink><br /><searchLink fieldCode="DE" term="%22Statistical+power+analysis%22">Statistical power analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Statistical+models%22">Statistical models</searchLink><br /><searchLink fieldCode="DE" term="%22Drinking+%28Physiology%29%22">Drinking (Physiology)</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+communication%22">Animal communication</searchLink><br /><searchLink fieldCode="DE" term="%22Research+funding%22">Research funding</searchLink><br /><searchLink fieldCode="DE" term="%22T-test+%28Statistics%29%22">T-test (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Ultrasonics%22">Ultrasonics</searchLink><br /><searchLink fieldCode="DE" term="%22Body+weight%22">Body weight</searchLink><br /><searchLink fieldCode="DE" term="%22Sample+size+%28Statistics%29%22">Sample size (Statistics)</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme-linked+immunosorbent+assay%22">Enzyme-linked immunosorbent assay</searchLink><br /><searchLink fieldCode="DE" term="%22Two-way+analysis+of+variance%22">Two-way analysis of variance</searchLink><br /><searchLink fieldCode="DE" term="%22Estrogen%22">Estrogen</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+transcriptase+polymerase+chain+reaction%22">Reverse transcriptase polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Descriptive+statistics%22">Descriptive statistics</searchLink><br /><searchLink fieldCode="DE" term="%22Chi-squared+test%22">Chi-squared test</searchLink><br /><searchLink fieldCode="DE" term="%22Estradiol%22">Estradiol</searchLink><br /><searchLink fieldCode="DE" term="%22Rats%22">Rats</searchLink><br /><searchLink fieldCode="DE" term="%22Serum%22">Serum</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Reward+%28Psychology%29%22">Reward (Psychology)</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+experimentation%22">Animal experimentation</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+behavior%22">Animal behavior</searchLink><br /><searchLink fieldCode="DE" term="%22Statistics%22">Statistics</searchLink><br /><searchLink fieldCode="DE" term="%22Water%22">Water</searchLink><br /><searchLink fieldCode="DE" term="%22Data+analysis+software%22">Data analysis software</searchLink><br /><searchLink fieldCode="DE" term="%22Anthropometry%22">Anthropometry</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Dehydration%22">Dehydration</searchLink><br /><searchLink fieldCode="DE" term="%22Uterus%22">Uterus</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Purpose: Hormonal and hydration states are known to individually influence laryngeal physiology, yet their distinct and combined effects on vocal function remain poorly understood. This study investigated the interaction of reduced circulating estrogen, estradiol replacement, and mild systemic dehydration (DEHY) on ultrasonic vocalization (USV) in adult female rats. Method: Forty-eight Long–Evans rats were assigned to six groups (n = 8 per group) that differed in hormonal state (control, ovariectomized + estradiol pellet [OVX + Estradiol], OVX + placebo pellet) and hydration status (euhydration [EUHY], DEHY). USVs were recorded during a food-reward elicitation paradigm before hormonal (pellet) replacement, after hormonal replacement, and after 5 days of EUHY or DEHY. Dependent measures included USV number, duration, frequency range, and maximum power. Physiological measures (body weight, water intake, kidney renin expression, uterine weight) supported experimental manipulations. Results: OVX animals produced shorter duration and lower power USVs compared with controls, although total number and frequency range were largely preserved. Even after hormonal replacement, OVX + Estradiol animals emitted fewer USVs with shorter duration, narrower frequency range, and reduced power as compared with control animals. DEHY narrowed USV frequency range across hormonal groups and interacted with hormonal status to modulate USV number and duration. Conclusions: Both circulating estrogen and systemic hydration status selectively influenced USV profiles. Although statistically significant, USV changes were generally of small magnitude, warranting caution in interpretation. These findings highlight the nuanced and context-dependent nature of endocrine and hydration influences on vocalizations and establish a preclinical framework for investigating hormone–hydration interactions in laryngeal function. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Speech, Language & Hearing Research is the property of American Speech-Language-Hearing Association and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=ehh&AN=195295658
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1044/2026_JSLHR-25-00912
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 21
        StartPage: 2940
    Subjects:
      – SubjectFull: Data analysis
        Type: general
      – SubjectFull: Comparative studies
        Type: general
      – SubjectFull: Laryngeal physiology
        Type: general
      – SubjectFull: Biological models
        Type: general
      – SubjectFull: Statistical power analysis
        Type: general
      – SubjectFull: Statistical models
        Type: general
      – SubjectFull: Drinking (Physiology)
        Type: general
      – SubjectFull: Animal communication
        Type: general
      – SubjectFull: Research funding
        Type: general
      – SubjectFull: T-test (Statistics)
        Type: general
      – SubjectFull: Ultrasonics
        Type: general
      – SubjectFull: Body weight
        Type: general
      – SubjectFull: Sample size (Statistics)
        Type: general
      – SubjectFull: Enzyme-linked immunosorbent assay
        Type: general
      – SubjectFull: Two-way analysis of variance
        Type: general
      – SubjectFull: Estrogen
        Type: general
      – SubjectFull: Reverse transcriptase polymerase chain reaction
        Type: general
      – SubjectFull: Descriptive statistics
        Type: general
      – SubjectFull: Chi-squared test
        Type: general
      – SubjectFull: Estradiol
        Type: general
      – SubjectFull: Rats
        Type: general
      – SubjectFull: Serum
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Reward (Psychology)
        Type: general
      – SubjectFull: Animal experimentation
        Type: general
      – SubjectFull: Animal behavior
        Type: general
      – SubjectFull: Statistics
        Type: general
      – SubjectFull: Water
        Type: general
      – SubjectFull: Data analysis software
        Type: general
      – SubjectFull: Anthropometry
        Type: general
      – SubjectFull: Biomarkers
        Type: general
      – SubjectFull: Dehydration
        Type: general
      – SubjectFull: Uterus
        Type: general
    Titles:
      – TitleFull: Impact of Circulating Estrogen and Mild Systemic Dehydration on Ultrasonic Vocalizations in Female Rats.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Dwenger, Kaitlyn
      – PersonEntity:
          Name:
            NameFull: do Nascimento, Naila Cannes
      – PersonEntity:
          Name:
            NameFull: Bailey, Taylor W.
      – PersonEntity:
          Name:
            NameFull: Xie, Jun
      – PersonEntity:
          Name:
            NameFull: Cox, Abigail
      – PersonEntity:
          Name:
            NameFull: Schaser, Allison J.
      – PersonEntity:
          Name:
            NameFull: Sivasankara, M. Preeti
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 07
              Text: Jul2026
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 10924388
          Numbering:
            – Type: volume
              Value: 69
            – Type: issue
              Value: 7
          Titles:
            – TitleFull: Journal of Speech, Language & Hearing Research
              Type: main
ResultId 1