Bibliographic Details
| Title: |
花生种子不同发育阶段的转录因子调控网络鉴定. |
| Alternate Title: |
Identification of regulatory networks of transcription factors at different seed developmental stages in peanut. |
| Authors: |
孙志辉1 20232015038@stu.scau.edu.cn, 鲁清1, 李海芬1, 黄硌1, 李少雄1, 陈小平1, 王润风1, 余倩霞1, 徐杨玉2, 洪彦彬1, 王键宽3, 汪文毅4, 刘浩1 liuhao@gdaas.cn |
| Source: |
Chinese Journal of Oil Crop Sciences. 2025, Vol. 47 Issue 5, p1108-1122. 15p. |
| Subject Terms: |
*Transcription factors, *Seed development, *Cell metabolism, *Transcriptomes, *Lipid synthesis, *Gene regulatory networks, *Peanuts, *Gene expression |
| Abstract (English): |
To comprehensively analyze transcription factor regulatory network during peanut seeds development, this study first measured phenotypic data of seeds and pods at 3 different developmental stages (5 d, 25 d, and 45 d) after fruit needle entered to soil. Subsequently, transcriptome sequencing was performed for gene expression analysis, identifying a total of 71 044 expressed genes, of which 5198 were newly identified transcripts. The study identified 5159 (25 vs 5), 7714 (45 vs 5), and 1712 (45 vs 25) differentially expressed genes at the 3 different developmental stages. Differential genes were studied by analyzing their involvements on biological metabolic pathways. Results showed that the most metabolic pathways focused on 25 d-seeds after fruit needle entering the soil. When 45 d after needle entering, the number of metabolic pathways decreased, but oil synthesis was becoming dominant. Based on differential gene profile, 544 transcription factors were screened, and a transcriptional regulatory network was constructed, which was mainly involved in important pathways related to seed development, such as plant hormone signal transduction, circadian rhythm regulation, and MAPK signaling pathways. Further research showed that 18 transcription factors were specifically highly expressed in seeds, and were positively correlated with developmental rate of the seeds in terms of oil accumulation-related transcription factors family ABI, NFYB, and WRI, which were verified by qPCR. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
为了对花生种子发育过程中的转录因子调控网络进行全面解析, 本研究首先对花生果针人土后3个不同 发育时期(5 d、25 d、45 d)的种子与荚果进行表型数据测定。随后利用转录组测序进行基因表达量分析, 共检测到 71 044个基因, 其中5198个为新鉴定的转录本。本研究分别于3个不同发育时期鉴定到5159(25vs5)、7714 (45vS5)、1712(45vS25)个差异表达基因。差异基因参与的生物学代谢通路富集分析表明, 果针人土后25 d的花生 种子涉及的代谢通路最多, 而果针人土后45 d的花生种子中代谢通路减少, 油脂合成占主导地位。此外, 基于差异 基因谱共筛选出544个转录因子, 并以此构建了转录调控网络, 结果表明这些转录调控网络主要涉及植物激素信号 转导、昼夜节律调控、MAPK信号途径等种子发育相关的重要途径。进一步的研究表明, 18个转录因子在种子内特 异髙表达, 且与种子油脂积累相关的转录因子家族ABI、NFYB与WRI的基因表达趋势与种子发育速率呈正相关 性, 并用qPCR进行验证。本研究基于3个不同发育时期的转录组测序数据构建了花生种子基因表达谱与转录因子 互作网络, 为探究花生种子发育的分子调控机制提供了参考. [ABSTRACT FROM AUTHOR] |
| Database: |
Energy & Power Source |
