Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii.
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| Title: | Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii. |
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| Authors: | Owoade, Ademola1 (AUTHOR), Alshami, Ali S.1 (AUTHOR) ali.alshami@und.edu, Sparling, Richard2 (AUTHOR), Levin, David B.3 (AUTHOR), Hay, Ryan S.2,3 (AUTHOR) |
| Source: | Biofuels, Bioproducts & Biorefining. Nov2025, Vol. 19 Issue 6, p1611-1621. 11p. |
| Subject Terms: | *Clostridium, *Titanium compounds, *Metabolites, *Ethanol fuel industry, *Electroactive substances, *Reducing agents |
| Abstract: | Interest in the microbial production of bioethanol from renewable sources has prompted efforts to improve its production in homoacetogenic bacteria. It has been suggested that a strongly reducing environment might support the formation of more reduced products. This study investigated the effects of titanium(III)nitrilotriacetic acid (Ti(III)NTA), a highly electronegative redox mediator, on Clostridium ljungdahlii cultures grown in fructose‐based media with either an N2 or a 20% CO2/80% H2 atmosphere, and in autotrophic cultures with 20% CO2/80% H2. An atmosphere containing a 4:1 H2:CO2 ratio, rather than the usual 3:1 ratio required for autotrophic acetate production in C. ljungdahlii, was used to determine the effect of excess H2 electrons on autotrophic versus mixotrophic fermentation. Excess H2 did not affect the metabolic end‐product profiles observed but the use of Ti(III)NTA resulted in increased production of reduced metabolites such as ethanol, 2,3‐butanediol and lactic acid beyond those reported when CO was used as the sole carbon and energy source. These results reveal a possible correlation between the redox potential of supplied electrons and their metabolic effect. The electronegativity of supplied electrons is crucial, as electrons with sufficient redox potential to reduce ferredoxin gave the observed result. [ABSTRACT FROM AUTHOR] |
| Database: | Energy & Power Source |
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| Abstract: | Interest in the microbial production of bioethanol from renewable sources has prompted efforts to improve its production in homoacetogenic bacteria. It has been suggested that a strongly reducing environment might support the formation of more reduced products. This study investigated the effects of titanium(III)nitrilotriacetic acid (Ti(III)NTA), a highly electronegative redox mediator, on Clostridium ljungdahlii cultures grown in fructose‐based media with either an N2 or a 20% CO2/80% H2 atmosphere, and in autotrophic cultures with 20% CO2/80% H2. An atmosphere containing a 4:1 H2:CO2 ratio, rather than the usual 3:1 ratio required for autotrophic acetate production in C. ljungdahlii, was used to determine the effect of excess H2 electrons on autotrophic versus mixotrophic fermentation. Excess H2 did not affect the metabolic end‐product profiles observed but the use of Ti(III)NTA resulted in increased production of reduced metabolites such as ethanol, 2,3‐butanediol and lactic acid beyond those reported when CO was used as the sole carbon and energy source. These results reveal a possible correlation between the redox potential of supplied electrons and their metabolic effect. The electronegativity of supplied electrons is crucial, as electrons with sufficient redox potential to reduce ferredoxin gave the observed result. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 1932104X |
| DOI: | 10.1002/bbb.2749 |
