Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii.
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| Title: | Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii. |
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| Authors: | Owoade, Ademola1 (AUTHOR), Alshami, Ali S.1 (AUTHOR) ali.alshami@und.edu, Sparling, Richard2 (AUTHOR), Levin, David B.3 (AUTHOR), Hay, Ryan S.2,3 (AUTHOR) |
| Source: | Biofuels, Bioproducts & Biorefining. Nov2025, Vol. 19 Issue 6, p1611-1621. 11p. |
| Subject Terms: | *Clostridium, *Titanium compounds, *Metabolites, *Ethanol fuel industry, *Electroactive substances, *Reducing agents |
| Abstract: | Interest in the microbial production of bioethanol from renewable sources has prompted efforts to improve its production in homoacetogenic bacteria. It has been suggested that a strongly reducing environment might support the formation of more reduced products. This study investigated the effects of titanium(III)nitrilotriacetic acid (Ti(III)NTA), a highly electronegative redox mediator, on Clostridium ljungdahlii cultures grown in fructose‐based media with either an N2 or a 20% CO2/80% H2 atmosphere, and in autotrophic cultures with 20% CO2/80% H2. An atmosphere containing a 4:1 H2:CO2 ratio, rather than the usual 3:1 ratio required for autotrophic acetate production in C. ljungdahlii, was used to determine the effect of excess H2 electrons on autotrophic versus mixotrophic fermentation. Excess H2 did not affect the metabolic end‐product profiles observed but the use of Ti(III)NTA resulted in increased production of reduced metabolites such as ethanol, 2,3‐butanediol and lactic acid beyond those reported when CO was used as the sole carbon and energy source. These results reveal a possible correlation between the redox potential of supplied electrons and their metabolic effect. The electronegativity of supplied electrons is crucial, as electrons with sufficient redox potential to reduce ferredoxin gave the observed result. [ABSTRACT FROM AUTHOR] |
| Database: | Energy & Power Source |
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| Header | DbId: enr DbLabel: Energy & Power Source An: 189332531 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Owoade%2C+Ademola%22">Owoade, Ademola</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alshami%2C+Ali+S%2E%22">Alshami, Ali S.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ali.alshami@und.edu</i><br /><searchLink fieldCode="AR" term="%22Sparling%2C+Richard%22">Sparling, Richard</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Levin%2C+David+B%2E%22">Levin, David B.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hay%2C+Ryan+S%2E%22">Hay, Ryan S.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biofuels%2C+Bioproducts+%26+Biorefining%22">Biofuels, Bioproducts & Biorefining</searchLink>. Nov2025, Vol. 19 Issue 6, p1611-1621. 11p. – Name: Subject Label: Subject Terms Group: Su Data: *<searchLink fieldCode="DE" term="%22Clostridium%22">Clostridium</searchLink><br />*<searchLink fieldCode="DE" term="%22Titanium+compounds%22">Titanium compounds</searchLink><br />*<searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink><br />*<searchLink fieldCode="DE" term="%22Ethanol+fuel+industry%22">Ethanol fuel industry</searchLink><br />*<searchLink fieldCode="DE" term="%22Electroactive+substances%22">Electroactive substances</searchLink><br />*<searchLink fieldCode="DE" term="%22Reducing+agents%22">Reducing agents</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Interest in the microbial production of bioethanol from renewable sources has prompted efforts to improve its production in homoacetogenic bacteria. It has been suggested that a strongly reducing environment might support the formation of more reduced products. This study investigated the effects of titanium(III)nitrilotriacetic acid (Ti(III)NTA), a highly electronegative redox mediator, on Clostridium ljungdahlii cultures grown in fructose‐based media with either an N2 or a 20% CO2/80% H2 atmosphere, and in autotrophic cultures with 20% CO2/80% H2. An atmosphere containing a 4:1 H2:CO2 ratio, rather than the usual 3:1 ratio required for autotrophic acetate production in C. ljungdahlii, was used to determine the effect of excess H2 electrons on autotrophic versus mixotrophic fermentation. Excess H2 did not affect the metabolic end‐product profiles observed but the use of Ti(III)NTA resulted in increased production of reduced metabolites such as ethanol, 2,3‐butanediol and lactic acid beyond those reported when CO was used as the sole carbon and energy source. These results reveal a possible correlation between the redox potential of supplied electrons and their metabolic effect. The electronegativity of supplied electrons is crucial, as electrons with sufficient redox potential to reduce ferredoxin gave the observed result. [ABSTRACT FROM AUTHOR] |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=enr&AN=189332531 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/bbb.2749 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1611 Subjects: – SubjectFull: Clostridium Type: general – SubjectFull: Titanium compounds Type: general – SubjectFull: Metabolites Type: general – SubjectFull: Ethanol fuel industry Type: general – SubjectFull: Electroactive substances Type: general – SubjectFull: Reducing agents Type: general Titles: – TitleFull: Extracellular electron supply from titanium(III)nitrilotriacetic acid promotes production of reduced fermentation end products in Clostridium ljungdahlii. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Owoade, Ademola – PersonEntity: Name: NameFull: Alshami, Ali S. – PersonEntity: Name: NameFull: Sparling, Richard – PersonEntity: Name: NameFull: Levin, David B. – PersonEntity: Name: NameFull: Hay, Ryan S. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 11 Text: Nov2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 1932104X Numbering: – Type: volume Value: 19 – Type: issue Value: 6 Titles: – TitleFull: Biofuels, Bioproducts & Biorefining Type: main |
| ResultId | 1 |
