Expression level and spatial distribution of opsin gene in Oratosquilla oratoria compound eye.
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| Title: | Expression level and spatial distribution of opsin gene in Oratosquilla oratoria compound eye. |
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| Authors: | Lou, Fangrui1 (AUTHOR) lfr199202@163.com, Duan, Xiaowen1 (AUTHOR), Zhou, Jiaoli1 (AUTHOR), Ding, Yue1 (AUTHOR), Han, Zhiqiang2 (AUTHOR) d6339124@163.com |
| Source: | Journal of Oceanology & Limnology. Jan2026, Vol. 44 Issue 1, p340-356. 17p. |
| Subject Terms: | *Stomatopoda, *Visual pigments, *Photoreceptors, *Optical polarization, *RNA sequencing |
| Abstract: | Due to the unique microstructure and diverse opsin genes of the trinocular compound eye, stomatopoda possess an extraordinary ability to perceive multiple properties of light. They not only can detect natural light (NL) and linearly polarized light (LPL), but also are the only animals capable of recognizing circularly polarized light (CPL). Here, we integrated single-cell RNA sequencing, previously published Illumina data, and in-situ hybridization (ISH) to quantify and localize functional opsin genes in Oratosquilla oratoria, a common stomatopoda species in the China Sea. A total of high-quality 31 777 cells were captured for the first time in the O. oratoria compound eye, which were classified into 25 cell subpopulations, and hypothesized that cluster 22 is a critical cell subpopulation responsible for light (whether NL, LPL, or CPL) response in O. oratoria. Furthermore, we propose that the long-wavelength-sensitive opsin gene (lws) gene family, retinol dehydrogenase (rdh), voltage-gated ion channel (vgic), arrestin (arr), and myosin (myo) collectively mediate the light response in O. oratoria. Considering that very few vision-related opsin genes show differential expression in right-handed CPL (RCPL) -vs.- dark (DL), which provides additional evidence that stomatopoda cannot recognize RCPL. Meanwhile, we believe that UV-stimulated scaffold protein A (uvssa) and red pigment concentrating hormone (rpch) play special contributions in the left-handed CPL (LCPL) environment response. ISH revealing that 16 lws, 6 middle-wavelength-sensitive (mws), and 2 ultraviolet (uv) opsin genes were expressed in the photoreceptors of the O. oratoria compound eye. Although the inability to determine the functional types of cell subpopulations limits the resolution of opsin genes, these findings systematically elucidate the specific expression patterns of opsin genes in O. oratoria and represent a significant step toward refining the visual ecological theory of O. oratoria and other stomatopod species. [ABSTRACT FROM AUTHOR] |
| Database: | Energy & Power Source |
| Abstract: | Due to the unique microstructure and diverse opsin genes of the trinocular compound eye, stomatopoda possess an extraordinary ability to perceive multiple properties of light. They not only can detect natural light (NL) and linearly polarized light (LPL), but also are the only animals capable of recognizing circularly polarized light (CPL). Here, we integrated single-cell RNA sequencing, previously published Illumina data, and in-situ hybridization (ISH) to quantify and localize functional opsin genes in Oratosquilla oratoria, a common stomatopoda species in the China Sea. A total of high-quality 31 777 cells were captured for the first time in the O. oratoria compound eye, which were classified into 25 cell subpopulations, and hypothesized that cluster 22 is a critical cell subpopulation responsible for light (whether NL, LPL, or CPL) response in O. oratoria. Furthermore, we propose that the long-wavelength-sensitive opsin gene (lws) gene family, retinol dehydrogenase (rdh), voltage-gated ion channel (vgic), arrestin (arr), and myosin (myo) collectively mediate the light response in O. oratoria. Considering that very few vision-related opsin genes show differential expression in right-handed CPL (RCPL) -vs.- dark (DL), which provides additional evidence that stomatopoda cannot recognize RCPL. Meanwhile, we believe that UV-stimulated scaffold protein A (uvssa) and red pigment concentrating hormone (rpch) play special contributions in the left-handed CPL (LCPL) environment response. ISH revealing that 16 lws, 6 middle-wavelength-sensitive (mws), and 2 ultraviolet (uv) opsin genes were expressed in the photoreceptors of the O. oratoria compound eye. Although the inability to determine the functional types of cell subpopulations limits the resolution of opsin genes, these findings systematically elucidate the specific expression patterns of opsin genes in O. oratoria and represent a significant step toward refining the visual ecological theory of O. oratoria and other stomatopod species. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 20965508 |
| DOI: | 10.1007/s00343-025-4305-z |
