Sericin stabilized emulgel for improving therapeutic efficacy of quercetin in treatment of diabetic wound healing.
Saved in:
| Title: | Sericin stabilized emulgel for improving therapeutic efficacy of quercetin in treatment of diabetic wound healing. |
|---|---|
| Authors: | Burud, Asmita1 (AUTHOR), Galatage, Sunil2 (AUTHOR) gsunil201288@gmail.com, Manjappa, Arehalli2 (AUTHOR), Salawi, Ahmad3 (AUTHOR), Nadaf, Sameer4 (AUTHOR) sam.nadaf@rediffmail.com, Holam, Machindra1 (AUTHOR), Harale, Swapnil1 (AUTHOR), Kumbhar, Ravindra5 (AUTHOR), Peram, Malleswara Rao6 (AUTHOR), Suryadevara, Vidyadhara6 (AUTHOR) |
| Source: | Journal of Dispersion Science & Technology. 2026, Vol. 47 Issue 7, p1400-1420. 21p. |
| Subject Terms: | *Quercetin, *Colloidal stability, *Zeta potential, *Treatment effectiveness, *Olive oil, *In vitro toxicity testing, *Diabetic foot |
| Abstract: | Emulsion stability is vital for effectiveness, ensuring uniform distribution of droplets and preventing phase separation. Instability can lead to inconsistent performance and reduced therapeutic benefits. Thus, our research endeavors were directed toward the design and characterization of a sericin (SN) stabilized quercetin (QRN) emulgel (EG), specifically tailored for diabetic wound healing. Employing a two-factor, 3-level factorial design SN-stabilized QRN emulgel (QSEG) was prepared and the influence of varying percentages of olive oil and SN on zeta potential, globule size, and entrapment efficiency (%) was scrutinized. The optimized QRN emulgel (QSEG), composed of 10% oil and 5% SN (QSE-6), showcased a diminutive globule size of 809.2 ± 21.3 nm alongside an elevated zeta potential of −42.9 ± 3.79 mV. Submicron-sized spherical globules were observed without any signs of coalescence, confirmed by surface morphology analysis. FTIR showed compatibility, while DSC and XRD confirmed that QRN was amorphized and evenly dispersed within the emulgel. In-vitro scratch assays manifested reduced intercellular distances and wound closure, indicative of potent wound healing attributes of QSEG. QSEG demonstrated notable antibacterial efficacy against Escherichia coli (E. coli), with minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of 3.125 ± 0.4 and 6.25 ± 1.2 μg/mL, respectively, surpassing that against Staphylococcus aureus (S.aureus). Cytotoxicity assays evidenced diminished toxicity toward fibroblast cells. Furthermore, in-vivo wound healing studies unveiled significant (p ˂ 0.001) enhancements with QSEG compared to QRN-treated and control groups. Our findings underscore SN's role as a natural stabilizer, synergistically enhancing QSEG's diabetic wound healing potential. Conclusively, QSEG emerges as a promising alternative to conventional diabetic wound healing treatments, offering inherent medicinal benefits. [ABSTRACT FROM AUTHOR] |
| Database: | Energy & Power Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| Abstract: | Emulsion stability is vital for effectiveness, ensuring uniform distribution of droplets and preventing phase separation. Instability can lead to inconsistent performance and reduced therapeutic benefits. Thus, our research endeavors were directed toward the design and characterization of a sericin (SN) stabilized quercetin (QRN) emulgel (EG), specifically tailored for diabetic wound healing. Employing a two-factor, 3-level factorial design SN-stabilized QRN emulgel (QSEG) was prepared and the influence of varying percentages of olive oil and SN on zeta potential, globule size, and entrapment efficiency (%) was scrutinized. The optimized QRN emulgel (QSEG), composed of 10% oil and 5% SN (QSE-6), showcased a diminutive globule size of 809.2 ± 21.3 nm alongside an elevated zeta potential of −42.9 ± 3.79 mV. Submicron-sized spherical globules were observed without any signs of coalescence, confirmed by surface morphology analysis. FTIR showed compatibility, while DSC and XRD confirmed that QRN was amorphized and evenly dispersed within the emulgel. In-vitro scratch assays manifested reduced intercellular distances and wound closure, indicative of potent wound healing attributes of QSEG. QSEG demonstrated notable antibacterial efficacy against Escherichia coli (E. coli), with minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of 3.125 ± 0.4 and 6.25 ± 1.2 μg/mL, respectively, surpassing that against Staphylococcus aureus (S.aureus). Cytotoxicity assays evidenced diminished toxicity toward fibroblast cells. Furthermore, in-vivo wound healing studies unveiled significant (p ˂ 0.001) enhancements with QSEG compared to QRN-treated and control groups. Our findings underscore SN's role as a natural stabilizer, synergistically enhancing QSEG's diabetic wound healing potential. Conclusively, QSEG emerges as a promising alternative to conventional diabetic wound healing treatments, offering inherent medicinal benefits. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 01932691 |
| DOI: | 10.1080/01932691.2024.2444980 |
