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How Fast Do Mitochondria Work? A Kinetic Study of Oxidative Phosphorylation

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Title: How Fast Do Mitochondria Work? A Kinetic Study of Oxidative Phosphorylation
Language: English
Authors: Ce´sar Pastrana-Pineda, Luis J. Melgoza-Rami´rez, Erandi M. Islas-Flores, Wendy L. Go´mez-Monsiva´is, Jero´nimo Gonce, Noe´ Lo´pez, Jose´ B. Limo´n-Gonza´lez, Jose´ S. Rodri´guez-Zavala, Emma Saavedra, Javier A. Belmont-Di´az (ORCID 0000-0003-0125-0223)
Source: Journal of Chemical Education. 2025 102(7):2866-2872.
Availability: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Peer Reviewed: Y
Page Count: 7
Publication Date: 2025
Document Type: Journal Articles
Reports - Descriptive
Descriptors: Science Instruction, Science Laboratories, Biochemistry, Energy, Metabolism, Fundamental Concepts, Cytology, Kinetics
DOI: 10.1021/acs.jchemed.4c00992
ISSN: 0021-9584
1938-1328
Abstract: The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation of carbohydrates. In this laboratory teaching experiment, we conducted basic respirometry experiments that allowed students to collect, process, and interpret data on mitochondrial metabolism; their challenge was to calculate the number of ATP molecules that mitochondria inside a cell can synthesize per second. The goal was to introduce basic concepts of bioenergetics and metabolism. The pedagogical objective was to assess whether understanding how a metabolic flux is experimentally evaluated and processing the resulting data could enhance students' knowledge of oxidative phosphorylation and its relevance to the cell. The models used in the respirometry experiments include a human breast cancer cell line, a nonmalignant mouse myoblast cell line, and "Trypanosoma cruzi," a homoflagellate parasite that causes Chagas disease and has the peculiarity of having a single mitochondrion.
Abstractor: As Provided
Entry Date: 2025
Accession Number: EJ1476696
Database: ERIC
FullText Text:
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  Data: <searchLink fieldCode="AR" term="%22Ce´sar+Pastrana-Pineda%22">Ce´sar Pastrana-Pineda</searchLink><br /><searchLink fieldCode="AR" term="%22Luis+J%2E+Melgoza-Rami´rez%22">Luis J. Melgoza-Rami´rez</searchLink><br /><searchLink fieldCode="AR" term="%22Erandi+M%2E+Islas-Flores%22">Erandi M. Islas-Flores</searchLink><br /><searchLink fieldCode="AR" term="%22Wendy+L%2E+Go´mez-Monsiva´is%22">Wendy L. Go´mez-Monsiva´is</searchLink><br /><searchLink fieldCode="AR" term="%22Jero´nimo+Gonce%22">Jero´nimo Gonce</searchLink><br /><searchLink fieldCode="AR" term="%22Noe´+Lo´pez%22">Noe´ Lo´pez</searchLink><br /><searchLink fieldCode="AR" term="%22Jose´+B%2E+Limo´n-Gonza´lez%22">Jose´ B. Limo´n-Gonza´lez</searchLink><br /><searchLink fieldCode="AR" term="%22Jose´+S%2E+Rodri´guez-Zavala%22">Jose´ S. Rodri´guez-Zavala</searchLink><br /><searchLink fieldCode="AR" term="%22Emma+Saavedra%22">Emma Saavedra</searchLink><br /><searchLink fieldCode="AR" term="%22Javier+A%2E+Belmont-Di´az%22">Javier A. Belmont-Di´az</searchLink> (ORCID <externalLink term="https://orcid.org/0000-0003-0125-0223">0000-0003-0125-0223</externalLink>)
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  Data: <searchLink fieldCode="SO" term="%22Journal+of+Chemical+Education%22"><i>Journal of Chemical Education</i></searchLink>. 2025 102(7):2866-2872.
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  Data: Division of Chemical Education, Inc. and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
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  Data: 10.1021/acs.jchemed.4c00992
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  Data: 0021-9584<br />1938-1328
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  Data: The study of energy metabolism is fundamental in multiple disciplines, such as biochemistry, bioengineering, biomedicine, etc. Traditionally, in high school and college level courses, students are taught energy metabolism (glycolysis, Krebs cycle, and oxidative phosphorylation) through diagrams of metabolic pathways that depict the transformation of carbohydrates. In this laboratory teaching experiment, we conducted basic respirometry experiments that allowed students to collect, process, and interpret data on mitochondrial metabolism; their challenge was to calculate the number of ATP molecules that mitochondria inside a cell can synthesize per second. The goal was to introduce basic concepts of bioenergetics and metabolism. The pedagogical objective was to assess whether understanding how a metabolic flux is experimentally evaluated and processing the resulting data could enhance students' knowledge of oxidative phosphorylation and its relevance to the cell. The models used in the respirometry experiments include a human breast cancer cell line, a nonmalignant mouse myoblast cell line, and "Trypanosoma cruzi," a homoflagellate parasite that causes Chagas disease and has the peculiarity of having a single mitochondrion.
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  Data: 2025
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  Data: EJ1476696
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