Development of a Green Fluorescent Protein-Based Laboratory Curriculum
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| Title: | Development of a Green Fluorescent Protein-Based Laboratory Curriculum |
|---|---|
| Language: | English |
| Authors: | Larkin, Patrick D., Hartberg, Yasha |
| Source: | Biochemistry and Molecular Biology Education. Jan 2005 33(1):41-45. |
| Availability: | John Wiley & Sons, Inc. Subscription Department, 111 River Street, Hoboken, NJ 07030-5774. Tel: 800-825-7550; Tel: 201-748-6645; Fax: 201-748-6021; e-mail: subinfo@wiley.com; Web site: https://secure.interscience.wiley.com/cgi-bin/jhome/112782101 |
| Peer Reviewed: | Y |
| Page Count: | 5 |
| Publication Date: | 2005 |
| Intended Audience: | Teachers |
| Document Type: | Guides - Classroom - Teacher Journal Articles Reports - Descriptive |
| Education Level: | Higher Education |
| Descriptors: | Science Laboratories, Biochemistry, Molecular Biology, Cytology, Genetics, Microbiology, Surveys, Teaching Methods, Curriculum Design |
| DOI: | 10.1002/bmb.2005.494033010417 |
| ISSN: | 1470-8175 |
| Abstract: | A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics. |
| Abstractor: | Author |
| Entry Date: | 2007 |
| Accession Number: | EJ759020 |
| Database: | ERIC |
| Abstract: | A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics. |
|---|---|
| ISSN: | 1470-8175 |
| DOI: | 10.1002/bmb.2005.494033010417 |
