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Development of a Green Fluorescent Protein-Based Laboratory Curriculum

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Title: Development of a Green Fluorescent Protein-Based Laboratory Curriculum
Language: English
Authors: Larkin, Patrick D., Hartberg, Yasha
Source: Biochemistry and Molecular Biology Education. Jan 2005 33(1):41-45.
Availability: John Wiley & Sons, Inc. Subscription Department, 111 River Street, Hoboken, NJ 07030-5774. Tel: 800-825-7550; Tel: 201-748-6645; Fax: 201-748-6021; e-mail: subinfo@wiley.com; Web site: https://secure.interscience.wiley.com/cgi-bin/jhome/112782101
Peer Reviewed: Y
Page Count: 5
Publication Date: 2005
Intended Audience: Teachers
Document Type: Guides - Classroom - Teacher
Journal Articles
Reports - Descriptive
Education Level: Higher Education
Descriptors: Science Laboratories, Biochemistry, Molecular Biology, Cytology, Genetics, Microbiology, Surveys, Teaching Methods, Curriculum Design
DOI: 10.1002/bmb.2005.494033010417
ISSN: 1470-8175
Abstract: A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics.
Abstractor: Author
Entry Date: 2007
Accession Number: EJ759020
Database: ERIC
FullText Text:
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  Data: A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics.
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