Expresión de los receptores de complemento CR3 y manosa MRC1 en neutrófilos humanos infectados con actinomicetos.

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Bibliographic Details
Title:	Expresión de los receptores de complemento CR3 y manosa MRC1 en neutrófilos humanos infectados con actinomicetos.
Alternate Title:	Expression of complement CR3 and mannose MRC1 receptors in human neutrophils infected with actinomycetes.
Authors:	Palma-Ramos, Alejandro¹, Argueta-Fuertes, Marco Antonio¹, Castrillón-Rivera, Laura Estela¹, Paredes-Rojas, Araceli¹, Santiago-Téllez, Alfonso¹, Muñoz-Duarte, Ana Rosa¹, Luna-Herrera, Julieta², Castañeda-Sánchez, Jorge Ismael¹ jcastanedas@correo.xoc.uam.mx
Source:	Dermatología Revista Mexicana. jul/ago2018, Vol. 62 Issue 4, p293-300. 8p.
Abstract (English):	BACKGROUND: Mycetoma is an infectious, granulomatous, chronic disease that affects the skin, subcutaneous cellular tissue and bone. It is caused by filamentous bacteria (actinomycetoma) and fungi (eumycetoma). In Mexico, the most frequent causative agent of actinomycetoma is Nocardia brasiliensis, followed by Actinomadura madurae. OBJECTIVE: To evaluate the expression of mannose (MRC1) and complement (CR3) receptors in human neutrophils infected with N. brasiliensis and A. madurae. MATERIAL AND METHOD: An experimental study performed from June to December 2017 was done in which human neutrophils from peripheral blood were infected with N. brasiliensis and A. madurae, the kinetics were followed post infection of 1, 2, 3, 4 and 5 hours. The total RNA was obtained for its subsequent retro-transcription, the quantification of the genes of the receptors was carried out by qPCR and the expression at the protein level was corroborated by confocal microscopy. RESULTS: In human neutrophils infected with N. brasiliensis and A. madurae, a high expression of the MRC1 receptor was detected which was increased from the first hours of infection with both strains, with respect to the CR3 receptor, we found that the neutrophils expressed a discreet way in early times of infection. CONCLUSION: Neutrophils infected with N. brasiliensis and A. madurae on express the mannose receptor MRC1; however, the levels of expression of complement receptor CR3 are low. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	ANTECEDENTES: El micetoma es una enfermedad infecciosa, granulomatosa, crónica, que afecta la piel, el tejido celular subcutáneo y el hueso; es causada por bacterias filamentosas (actinomicetoma) y hongos (eumicetoma). En México el agente causal más frecuente de actinomicetoma es Nocardia brasiliensis, seguido por Actinomadura madurae. OBJETIVO: Evaluar la expresión de los receptores manosa (MRC1) y de complemento (CR3) en neutrófilos humanos infectados con N. brasiliensis y A. madurae. MATERIAL Y MÉTODO: Estudio experimental efectuado de junio a diciembre de 2017 en el que se infectaron neutrófilos humanos obtenidos de sangre periférica con N. brasiliensis y A. madurae, se siguieron cinéticas posinfección de 1, 2, 3, 4 y 5 horas. Se obtuvo ARN total para su posterior retro-transcripción, la cuantificación de los genes de los receptores se realizó por qPCR y la expresión a nivel proteico se corroboró por microscopia confocal. RESULTADOS: En neutrófilos humanos infectados con N. brasiliensis y A. madurae se detectó alta expresión del receptor de MRC1 que se vio incrementado desde las primeras horas de infección con ambas cepas, respecto al receptor CR3 encontramos que los neutrófilos lo expresan de manera discreta en tiempos tempranos de la infección. CONCLUSIÓN: Los neutrófilos infectados con N. brasiliensis y A. madurae sobreexpresan el receptor de manosa MRC1; sin embargo, los niveles de expresión del receptor de complemento CR3 son bajos. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	BACKGROUND: Mycetoma is an infectious, granulomatous, chronic disease that affects the skin, subcutaneous cellular tissue and bone. It is caused by filamentous bacteria (actinomycetoma) and fungi (eumycetoma). In Mexico, the most frequent causative agent of actinomycetoma is Nocardia brasiliensis, followed by Actinomadura madurae. OBJECTIVE: To evaluate the expression of mannose (MRC1) and complement (CR3) receptors in human neutrophils infected with N. brasiliensis and A. madurae. MATERIAL AND METHOD: An experimental study performed from June to December 2017 was done in which human neutrophils from peripheral blood were infected with N. brasiliensis and A. madurae, the kinetics were followed post infection of 1, 2, 3, 4 and 5 hours. The total RNA was obtained for its subsequent retro-transcription, the quantification of the genes of the receptors was carried out by qPCR and the expression at the protein level was corroborated by confocal microscopy. RESULTS: In human neutrophils infected with N. brasiliensis and A. madurae, a high expression of the MRC1 receptor was detected which was increased from the first hours of infection with both strains, with respect to the CR3 receptor, we found that the neutrophils expressed a discreet way in early times of infection. CONCLUSION: Neutrophils infected with N. brasiliensis and A. madurae on express the mannose receptor MRC1; however, the levels of expression of complement receptor CR3 are low. [ABSTRACT FROM AUTHOR]
ISSN:	01854038