Bibliographic Details
| Title: |
EVALUACIÓN DEL ÍNDICE MITÓTICO DE MERISTEMOS RADICULARES DE ALLIUM CEPA "CEBOLLA" EXPUESTOS A DIFERENTES CONCENTRACIONES DE METOTREXATO. |
| Alternate Title: |
EVALUATION OF THE MITOTIC INDEX OF ROOT MERISTEMS OF ALLIUM CEPA "ONION" EXPOSED TO DIFFERENT CONCENTRATIONS OF METHOTREXATE. |
| Authors: |
Malca-Diaz, Jimmy Fernando rdiaz@usat.edu.pe, Incio-Granthon, Lic. Marianella Elizabeth, Ñique-Carbajal, Mtro. Cesar Armando, Rodríguez-Cruz, Mtra. Lisseth Dolores, Diaz-Manchay, Dra. Rosa Jeuna, Malca-Vásquez, Méd. Jimmy Alexander |
| Source: |
Medicina Naturista. jul-dic2020, Vol. 14 Issue 2, p117-122. 6p. |
| Subjects: |
ONIONS, TUKEY'S test, ANALYSIS of variance, CELL division, ROOT growth |
| Abstract (English): |
The objective was to evaluate the mitotic index of root meristems of Allium cepa, exposed to different concentrations of methotrexate. Study with quantitative and experimental approach. Onion bulbs were used, whose water was changed every 24 hours during a period of 7 days prior to the analysis, in order to induce the growth of the root meristem, establishing two types: the control group, that were the bulbs that continued with water, and the experimental group, consisting of the bulbs exposed to different concentrations of methotrexate solution (0.1, 1 and 2.5 mg/mL). Subsequently, the rootlets of both groups were fixed for the study of the mitotic index, determining 3 repetitions for each sample and coloring each unit of analysis with aceto-orcein staining. The data was processed with the SPSS version 21 software, performing a variance analysis (ANOVA) and the Tukey's test at 5% significance. It is concluded that as the concentration of methotrexate is higher, the rate of cell division of Allium cepa "onion" is lower, demonstrating its cytotoxicity. This indicates the inhibitory action of methotrexate on cell division. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
El objetivo fue evaluar el índice mitótico de meristemos radiculares de Allium cepa, expuestos a diferentes concentraciones de metotrexato. Estudio con enfoque cuantitativo y experimental. Se utilizaron bulbos de cebollas, a los cuales se les cambió de agua cada 24 horas durante un período de 7 días previos al análisis, con la finalidad de inducir el crecimiento del meristemo radicular, estableciéndose dos tipos: el grupo control, que fueron los bulbos que continuaron con agua, y el grupo experimental, constituido por los bulbos expuestos a diferentes concentraciones de solución de metotrexato (0,1, 1 y 2,5 mg/mL). Posteriormente, se fijaron las raicillas de ambos grupos para el estudio del índice mitótico, determinándose 3 repeticiones para cada muestra y coloreándose cada unidad de análisis con la tinción de orceína acética. Los datos se procesaron con el software SPSS versión 21, realizándose un análisis de varianza (ANOVA) y la prueba de Tukey al 5% de significancia. Se concluye que a medida que la concentración de metotrexato es mayor, la tasa de división celular de Allium cepa "cebolla", es menor, demostrando su citotoxicidad. Esto indica la acción inhibitoria del metotrexato sobre la división celular. [ABSTRACT FROM AUTHOR] |
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| Database: |
MedicLatina |
