EVALUACIÓN DEL ÍNDICE MITÓTICO DE MERISTEMOS RADICULARES DE ALLIUM CEPA "CEBOLLA" EXPUESTOS A DIFERENTES CONCENTRACIONES DE METOTREXATO.
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| Title: | EVALUACIÓN DEL ÍNDICE MITÓTICO DE MERISTEMOS RADICULARES DE ALLIUM CEPA "CEBOLLA" EXPUESTOS A DIFERENTES CONCENTRACIONES DE METOTREXATO. |
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| Alternate Title: | EVALUATION OF THE MITOTIC INDEX OF ROOT MERISTEMS OF ALLIUM CEPA "ONION" EXPOSED TO DIFFERENT CONCENTRATIONS OF METHOTREXATE. |
| Authors: | Malca-Diaz, Jimmy Fernando rdiaz@usat.edu.pe, Incio-Granthon, Lic. Marianella Elizabeth, Ñique-Carbajal, Mtro. Cesar Armando, Rodríguez-Cruz, Mtra. Lisseth Dolores, Diaz-Manchay, Dra. Rosa Jeuna, Malca-Vásquez, Méd. Jimmy Alexander |
| Source: | Medicina Naturista. jul-dic2020, Vol. 14 Issue 2, p117-122. 6p. |
| Subjects: | ONIONS, TUKEY'S test, ANALYSIS of variance, CELL division, ROOT growth |
| Abstract (English): | The objective was to evaluate the mitotic index of root meristems of Allium cepa, exposed to different concentrations of methotrexate. Study with quantitative and experimental approach. Onion bulbs were used, whose water was changed every 24 hours during a period of 7 days prior to the analysis, in order to induce the growth of the root meristem, establishing two types: the control group, that were the bulbs that continued with water, and the experimental group, consisting of the bulbs exposed to different concentrations of methotrexate solution (0.1, 1 and 2.5 mg/mL). Subsequently, the rootlets of both groups were fixed for the study of the mitotic index, determining 3 repetitions for each sample and coloring each unit of analysis with aceto-orcein staining. The data was processed with the SPSS version 21 software, performing a variance analysis (ANOVA) and the Tukey's test at 5% significance. It is concluded that as the concentration of methotrexate is higher, the rate of cell division of Allium cepa "onion" is lower, demonstrating its cytotoxicity. This indicates the inhibitory action of methotrexate on cell division. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | El objetivo fue evaluar el índice mitótico de meristemos radiculares de Allium cepa, expuestos a diferentes concentraciones de metotrexato. Estudio con enfoque cuantitativo y experimental. Se utilizaron bulbos de cebollas, a los cuales se les cambió de agua cada 24 horas durante un período de 7 días previos al análisis, con la finalidad de inducir el crecimiento del meristemo radicular, estableciéndose dos tipos: el grupo control, que fueron los bulbos que continuaron con agua, y el grupo experimental, constituido por los bulbos expuestos a diferentes concentraciones de solución de metotrexato (0,1, 1 y 2,5 mg/mL). Posteriormente, se fijaron las raicillas de ambos grupos para el estudio del índice mitótico, determinándose 3 repeticiones para cada muestra y coloreándose cada unidad de análisis con la tinción de orceína acética. Los datos se procesaron con el software SPSS versión 21, realizándose un análisis de varianza (ANOVA) y la prueba de Tukey al 5% de significancia. Se concluye que a medida que la concentración de metotrexato es mayor, la tasa de división celular de Allium cepa "cebolla", es menor, demostrando su citotoxicidad. Esto indica la acción inhibitoria del metotrexato sobre la división celular. [ABSTRACT FROM AUTHOR] |
| Copyright of Medicina Naturista is the property of Sociedad Europea de Medicina Naturista Clasica (Seccion Espanola) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 146519972 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: EVALUACIÓN DEL ÍNDICE MITÓTICO DE MERISTEMOS RADICULARES DE ALLIUM CEPA "CEBOLLA" EXPUESTOS A DIFERENTES CONCENTRACIONES DE METOTREXATO. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: EVALUATION OF THE MITOTIC INDEX OF ROOT MERISTEMS OF ALLIUM CEPA "ONION" EXPOSED TO DIFFERENT CONCENTRATIONS OF METHOTREXATE. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Malca-Diaz%2C+Jimmy+Fernando%22">Malca-Diaz, Jimmy Fernando</searchLink><i> rdiaz@usat.edu.pe</i><br /><searchLink fieldCode="AR" term="%22Incio-Granthon%2C+Lic%2E+Marianella+Elizabeth%22">Incio-Granthon, Lic. Marianella Elizabeth</searchLink><br /><searchLink fieldCode="AR" term="%22Ñique-Carbajal%2C+Mtro%2E+Cesar+Armando%22">Ñique-Carbajal, Mtro. Cesar Armando</searchLink><br /><searchLink fieldCode="AR" term="%22Rodríguez-Cruz%2C+Mtra%2E+Lisseth+Dolores%22">Rodríguez-Cruz, Mtra. Lisseth Dolores</searchLink><br /><searchLink fieldCode="AR" term="%22Diaz-Manchay%2C+Dra%2E+Rosa+Jeuna%22">Diaz-Manchay, Dra. Rosa Jeuna</searchLink><br /><searchLink fieldCode="AR" term="%22Malca-Vásquez%2C+Méd%2E+Jimmy+Alexander%22">Malca-Vásquez, Méd. Jimmy Alexander</searchLink> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Medicina+Naturista%22">Medicina Naturista</searchLink>. jul-dic2020, Vol. 14 Issue 2, p117-122. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22ONIONS%22">ONIONS</searchLink><br /><searchLink fieldCode="DE" term="%22TUKEY'S+test%22">TUKEY'S test</searchLink><br /><searchLink fieldCode="DE" term="%22ANALYSIS+of+variance%22">ANALYSIS of variance</searchLink><br /><searchLink fieldCode="DE" term="%22CELL+division%22">CELL division</searchLink><br /><searchLink fieldCode="DE" term="%22ROOT+growth%22">ROOT growth</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: The objective was to evaluate the mitotic index of root meristems of Allium cepa, exposed to different concentrations of methotrexate. Study with quantitative and experimental approach. Onion bulbs were used, whose water was changed every 24 hours during a period of 7 days prior to the analysis, in order to induce the growth of the root meristem, establishing two types: the control group, that were the bulbs that continued with water, and the experimental group, consisting of the bulbs exposed to different concentrations of methotrexate solution (0.1, 1 and 2.5 mg/mL). Subsequently, the rootlets of both groups were fixed for the study of the mitotic index, determining 3 repetitions for each sample and coloring each unit of analysis with aceto-orcein staining. The data was processed with the SPSS version 21 software, performing a variance analysis (ANOVA) and the Tukey's test at 5% significance. It is concluded that as the concentration of methotrexate is higher, the rate of cell division of Allium cepa "onion" is lower, demonstrating its cytotoxicity. This indicates the inhibitory action of methotrexate on cell division. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: El objetivo fue evaluar el índice mitótico de meristemos radiculares de Allium cepa, expuestos a diferentes concentraciones de metotrexato. Estudio con enfoque cuantitativo y experimental. Se utilizaron bulbos de cebollas, a los cuales se les cambió de agua cada 24 horas durante un período de 7 días previos al análisis, con la finalidad de inducir el crecimiento del meristemo radicular, estableciéndose dos tipos: el grupo control, que fueron los bulbos que continuaron con agua, y el grupo experimental, constituido por los bulbos expuestos a diferentes concentraciones de solución de metotrexato (0,1, 1 y 2,5 mg/mL). Posteriormente, se fijaron las raicillas de ambos grupos para el estudio del índice mitótico, determinándose 3 repeticiones para cada muestra y coloreándose cada unidad de análisis con la tinción de orceína acética. Los datos se procesaron con el software SPSS versión 21, realizándose un análisis de varianza (ANOVA) y la prueba de Tukey al 5% de significancia. Se concluye que a medida que la concentración de metotrexato es mayor, la tasa de división celular de Allium cepa "cebolla", es menor, demostrando su citotoxicidad. Esto indica la acción inhibitoria del metotrexato sobre la división celular. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Medicina Naturista is the property of Sociedad Europea de Medicina Naturista Clasica (Seccion Espanola) and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=146519972 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 6 StartPage: 117 Subjects: – SubjectFull: ONIONS Type: general – SubjectFull: TUKEY'S test Type: general – SubjectFull: ANALYSIS of variance Type: general – SubjectFull: CELL division Type: general – SubjectFull: ROOT growth Type: general Titles: – TitleFull: EVALUACIÓN DEL ÍNDICE MITÓTICO DE MERISTEMOS RADICULARES DE ALLIUM CEPA "CEBOLLA" EXPUESTOS A DIFERENTES CONCENTRACIONES DE METOTREXATO. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Malca-Diaz, Jimmy Fernando – PersonEntity: Name: NameFull: Incio-Granthon, Lic. Marianella Elizabeth – PersonEntity: Name: NameFull: Ñique-Carbajal, Mtro. Cesar Armando – PersonEntity: Name: NameFull: Rodríguez-Cruz, Mtra. Lisseth Dolores – PersonEntity: Name: NameFull: Diaz-Manchay, Dra. Rosa Jeuna – PersonEntity: Name: NameFull: Malca-Vásquez, Méd. Jimmy Alexander IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: jul-dic2020 Type: published Y: 2020 Identifiers: – Type: issn-print Value: 15763080 Numbering: – Type: volume Value: 14 – Type: issue Value: 2 Titles: – TitleFull: Medicina Naturista Type: main |
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