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Hidrolizado de Sericina "SeriC": Evaluación del potencial genotóxico en ensayos a corto plazo.

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Title: Hidrolizado de Sericina "SeriC": Evaluación del potencial genotóxico en ensayos a corto plazo.
Alternate Title: Sericin hydrolysate "SeriC": Assessment of genotoxic potential in short-term assays.
Authors: Villa Testa, Anabel1, Fuentes Wajner, Sarah1, Medina Blanco, Zuarmis1, Ruiz Barrcena, Adileidys2, Padrón Yaquis, Saúl1, Piloto Ferrer, Janet1 janet.piloto@cidem.cu
Source: Cuban Journal of Biological Sciences / Revista Cubana de Ciencias Biológicas. jul-dic2021, Vol. 9 Issue 2, p1-7. 7p.
Subjects: AMES test, SPINDLE apparatus, PROTEOLYTIC enzymes, DNA damage, SERICIN, PHENOL oxidase
Geographic Terms: CUBA
Abstract (English): The Sericulture in the world has been developed for more than 5,000 years and Cuba undertake this activity as a sustainable alternative for the development of products for the biomedical, biotechnological, cosmetic and textile industries. Sericin, as a natural protein, is susceptible to the action of proteolytic enzymes; therefore it is digestible, biocompatible and biodegradable. It has been reported that it can be used in the food and cosmeceutical area due to its properties such as antioxidant and antityrosinase activity. However, nowadays the information that is known about its potential damage to DNA is scarce. Hence, the aim of this study was to evaluate the genotoxic potential of the sericin hydrolysate SeriC for its subsequent incorporation into cosmeceutical formulations. In order to achieve this goal, two short-term tests were used, the in vitro Salmonella / microsome system (Ames test) and the in vivo model of micronucleus induction in mouse bone marrow. The results obtained in the Ames test showed that SeriC, at the concentrations tested, did not cause DNA damage. Likewise, the mouse bone marrow micronucleus induction test showed that SeriC, administered via oral, did not induce chromosomal damage or mitotic spindle damage in the erythropoietic tissue of treated animals. This was verified because an increase in the frequency of micronuclei was not observed in the doses used compared to the negative and untreated controls. These results show that the sericin hydrolysate SeriC is considered non-genotoxic under our evaluated conditions. Furthermore, it can be used as a safe raw material for its future inclusion in different formulations for the pharmaceutical, cosmetic and food industries. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): La Sericultura en el mundo data de más de 5 mil años y Cuba asume esta actividad como una alternativa sostenible para el desarrollo de productos destinados a las industrias biomédicas, biotecnológicas, cosméticas y textil. La sericina, por ser una proteína natural, es susceptible a la acción de enzimas proteolíticas, por lo tanto es digerible, biocompatible y biodegradable y se ha informado que puede ser aprovechada en el área de los alimentos y cosmecéutica debido a que posee propiedades como actividad antioxidante y antitirosinasa. Sin embargo, hasta la fecha muy poco se conoce sobre su potencial daño al ADN. Por tal motivo el objetivo del presente estudio fue evaluar el potencial genotóxico del Hidrolizado de sericina SeriC para su posterior incorporación en formulaciones cosmecéuticas. Para ello, se utilizaron dos ensayos a corto plazo, el sistema in vitro Salmonella/microsoma (Test de Ames) y el modelo in vivo de inducción de micronúcleos en médula ósea de ratón. Los resultados obtenidos en el test de Ames mostraron que SeriC a las concentraciones evaluadas no ocasionó daño al DNA. Igualmente el ensayo de inducción de micronúcleos en médula ósea de ratón mostró que Seric C administrado por vía oral no indujo daño cromosómico, ni sobre el huso mitótico en el tejido eritropoyético de los animales tratados, lo cual se corroboró al no observarse aumento de la frecuencia de micronúcleos en las dosis empleadas respecto a los controles negativos y no tratados. Estos resultados indican que el hidrolizado de sericina SeriC se considera no genotóxico en las condiciones evaluadas por lo que pudiera emplearse como materia prima segura para su futura inclusión en diferentes formulaciones para las industrias farmacéutica, cosmética y alimentaria. [ABSTRACT FROM AUTHOR]
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Database: MedicLatina
Description
Abstract:The Sericulture in the world has been developed for more than 5,000 years and Cuba undertake this activity as a sustainable alternative for the development of products for the biomedical, biotechnological, cosmetic and textile industries. Sericin, as a natural protein, is susceptible to the action of proteolytic enzymes; therefore it is digestible, biocompatible and biodegradable. It has been reported that it can be used in the food and cosmeceutical area due to its properties such as antioxidant and antityrosinase activity. However, nowadays the information that is known about its potential damage to DNA is scarce. Hence, the aim of this study was to evaluate the genotoxic potential of the sericin hydrolysate SeriC for its subsequent incorporation into cosmeceutical formulations. In order to achieve this goal, two short-term tests were used, the in vitro Salmonella / microsome system (Ames test) and the in vivo model of micronucleus induction in mouse bone marrow. The results obtained in the Ames test showed that SeriC, at the concentrations tested, did not cause DNA damage. Likewise, the mouse bone marrow micronucleus induction test showed that SeriC, administered via oral, did not induce chromosomal damage or mitotic spindle damage in the erythropoietic tissue of treated animals. This was verified because an increase in the frequency of micronuclei was not observed in the doses used compared to the negative and untreated controls. These results show that the sericin hydrolysate SeriC is considered non-genotoxic under our evaluated conditions. Furthermore, it can be used as a safe raw material for its future inclusion in different formulations for the pharmaceutical, cosmetic and food industries. [ABSTRACT FROM AUTHOR]
ISSN:2307695X