Bibliographic Details
| Title: |
Polimorfismos de nucleótido simple en hormonas asociadas al crecimiento muscular en ovinos criollos colombianos. |
| Alternate Title: |
Single nucleotide polymorphisms in hormones associated with muscle growth in colombian creole sheep. |
| Authors: |
Nicolás Sarmiento, Paul1 paulsarcar@bg.fcen.uba.ar, Fernando Ariza, Manuel2, Teresa Ortiz, Yurany3, Lorena Castro, Susan4 |
| Source: |
NOVA: Publicación Científica en Ciencias Biomédicas. ene-jun2023, Vol. 21 Issue 40, p57-74. 18p. |
| Subjects: |
SINGLE nucleotide polymorphisms, GENETIC polymorphisms, GENOTYPES, SOMATOTROPIN, POLYMERASE chain reaction |
| Abstract (English): |
Objective. To determine the Single Nucleotide Polymorphisms (SNPs) present in growth hormone (GH) and insulin like growth factor 1 (IGF-1) genes and their association with muscle growth in Colombian Creole hair sheep. Materials and methods. A population of 100 sheep was selected, from three different regions: Andean valleys, Piedemonte Llanero and Córdoba department, subjected to different production systems. Polymorphisms identification was determined by the Polymerase Chain Reaction (PCR) and Single Chain Conformation Polymorphism (SSCP) techniques. Results. AA, AB and BB genotypes were identified for these genes. Allele frequencies were defined for the GH and IGF-1 (IGFov-1, IGF1ov-2 and IGF1ov-3) markers of 58.9, 36.87, 53.76 and 56.81% for allele A, respectively, and 41.41, 63.13, 46.24 and 43.18% for allele B, respectively. Genotypic frequencies were also determined for each marker at the population level, calculated from the Hardy-Weinberg equilibrium with a Fis correlation analysis. Conclusion. The selected markers present a high level of homology in the selected population, and it was determined that there is a high percentage of heterozygous individuals based on the markers evaluated. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
Objetivo. Determinar Polimorfismos de Nucleótido Simple (SNPs) presentes en los genes de la Hormona del Crecimiento (HC) y del Factor de Crecimiento Semejante a la Insulina 1 (IGF-1) y su asociación con el crecimiento muscular en ovinos de pelo criollos colombianos. Materiales y métodos. Se seleccionó una población de 100 ovinos, de tres regiones diferentes: Valles interandinos, Piedemonte Llanero y departamento de Córdoba, sometidos a diferentes sistemas de producción. La identificación de polimorfismos en los genes se realizó mediante las técnicas de Reacción en Cadena de la Polimerasa (PCR) y de Polimorfismo Conformacional de Cadena Sencilla (SSCP). Resultados. Se identificaron los genotipos AA, AB y BB para dichos genes. Las frecuencias alélicas para los marcadores HC, IGF-1 (IGFov-1, IGF1ov-2 e IGF1ov-3) fueron de 58,9, 36,87, 53,76 y 56,81% para el alelo A, respectivamente, y de 41,41, 63,13, 46,24 y 43,18% para el alelo B, respectivamente. Asimismo, se determinaron las frecuencias genotípicas para cada marcador a nivel poblacional, calculado a partir del equilibrio de Hardy-Weinberg con un análisis de correlación Fis. Conclusión. Los marcadores seleccionados presentaron un alto nivel de homología en la población seleccionada, lográndose determinar que existe un alto porcentaje de individuos heterocigotos con base a los marcadores evaluados. [ABSTRACT FROM AUTHOR] |
|
Copyright of NOVA: Publicación Científica en Ciencias Biomédicas is the property of Universidad Colegio Mayor de Cundinamarca and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
MedicLatina |
