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Evaluación in vitro de la patogenicidad de los hongos aislados en la región de Urabá (Antioquia, Colombia) contra larvas de Aedes aegypti.

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Title: Evaluación in vitro de la patogenicidad de los hongos aislados en la región de Urabá (Antioquia, Colombia) contra larvas de Aedes aegypti.
Alternate Title: In vitro evaluation of the pathogenicity of fungi isolated from the Urabá region (Antioquia, Colombia) against Aedes aegypti larvae.
Authors: Andrés Machado-Agudelo, Dairon1, Alejandra García, María1, Rueda-Páramo, Manuel E.2, Lorena Cardona, Nadya1 nadya.cardona@udea.edu.co
Source: Biomédica: Revista del Instituto Nacional de Salud. dic2024, Vol. 44 Issue 4, p552-563. 12p.
Subjects: AEDES aegypti, ARBOVIRUS diseases, BIOLOGICAL control of mosquitoes, DISEASE vectors, BODIES of water
Abstract (English): Introduction. Aedes aegypti is an important vector of arboviral diseases like dengue among others. Traditional control strategies, such as the use of insecticides, have lost effectiveness due to the emergence of resistance in mosquito populations. Biological control and fungi applied for biocontrol are presented as viable and ecological alternatives. Objective. To evaluate in vitro pathogenicity of Trichoderma sp. isolates obtained from Urabá (Antioquia) on larvae of Ae. aegypti, and to determine the mean lethal concentration and mean lethal time of the most pathogenic isolate. Materials and methods. Using the sentinel larvae method of Ae. aegypti, fungi were isolated from water bodies in the Urabá region (Antioquia). The isolates were characterized morphologically and molecularly to determine their taxonomic identity. Pathogenicity tests were performed in vitro on Ae. aegypti larvae in the L2/L3 stages. Subsequently, a strain was selected to establish its mean lethal concentration and mean lethal time. Results. Trichoderma sp. strain AP-91 caused high mortality in larval populations of Aedes aegypti. We estimated a mean lethal concentration of 1.8 × 107 conidia/ml and a mean lethal time of 20.67 hours. Conclusion. The strain AP-91 showed potential for its use as biological control of Ae. aegypti, making it a suitable candidate for scale-up cultures applied to integrated vector management. This research suggests exploring compounds and enzymes produced by the AP-91 strain to understand better its pathogenicity. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Introducción. Aedes aegypti es un vector importante de enfermedades arbovirales como el dengue, entre otras. Las estrategias tradicionales de control, como el uso de insecticidas, han perdido eficacia debido a la aparición de resistencia en las poblaciones de mosquitos. El control biológico y el uso de hongos biocontroladores se presentan como alternativas viables y amigables con el medio ambiente. Objetivo. Evaluar la patogenicidad in vitro de aislamientos del género Trichoderma -obtenidos del Urabá antioqueño- sobre larvas de Ae. aegypti, y determinar la concentración letal media (CL50) y el tiempo letal medio (TL50) del más patógenZonómica. Se llevaron a cabo pruebas de patogenicidad in vitro utilizando larvas de Ae. aegypti en los estadios L2 y L3. Posteriormente, se seleccionó una cepa para determinar su concentración letal media y tiempo letal medio. Resultados. La cepa AP-91 de Trichoderma sp. causó porcentajes altos de mortalidad en poblaciones de larvas de Ae. aegypti. Se obtuvo una concentración letal media de 1,8 × 107 conidios/ml y un tiempo letal medio de 20,67 horas. Conclusión. La cepa AP-91 tiene potencial para el control biológico de Ae. aegypti y puede ser una candidata idónea para usar en el manejo integrado de vectores mediante su cultivo escalado. La investigación sugiere explorar los compuestos y enzimas producidos por esta cepa para comprender mejor su patogenicidad. [ABSTRACT FROM AUTHOR]
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Evaluación in vitro de la patogenicidad de los hongos aislados en la región de Urabá (Antioquia, Colombia) contra larvas de Aedes aegypti.
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  Data: In vitro evaluation of the pathogenicity of fungi isolated from the Urabá region (Antioquia, Colombia) against Aedes aegypti larvae.
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  Data: <searchLink fieldCode="AR" term="%22Andrés+Machado-Agudelo%2C+Dairon%22">Andrés Machado-Agudelo, Dairon</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Alejandra+García%2C+María%22">Alejandra García, María</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rueda-Páramo%2C+Manuel+E%2E%22">Rueda-Páramo, Manuel E.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lorena+Cardona%2C+Nadya%22">Lorena Cardona, Nadya</searchLink><relatesTo>1</relatesTo><i> nadya.cardona@udea.edu.co</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomédica%3A+Revista+del+Instituto+Nacional+de+Salud%22">Biomédica: Revista del Instituto Nacional de Salud</searchLink>. dic2024, Vol. 44 Issue 4, p552-563. 12p.
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  Data: <searchLink fieldCode="DE" term="%22AEDES+aegypti%22">AEDES aegypti</searchLink><br /><searchLink fieldCode="DE" term="%22ARBOVIRUS+diseases%22">ARBOVIRUS diseases</searchLink><br /><searchLink fieldCode="DE" term="%22BIOLOGICAL+control+of+mosquitoes%22">BIOLOGICAL control of mosquitoes</searchLink><br /><searchLink fieldCode="DE" term="%22DISEASE+vectors%22">DISEASE vectors</searchLink><br /><searchLink fieldCode="DE" term="%22BODIES+of+water%22">BODIES of water</searchLink>
– Name: Abstract
  Label: Abstract (English)
  Group: Ab
  Data: Introduction. Aedes aegypti is an important vector of arboviral diseases like dengue among others. Traditional control strategies, such as the use of insecticides, have lost effectiveness due to the emergence of resistance in mosquito populations. Biological control and fungi applied for biocontrol are presented as viable and ecological alternatives. Objective. To evaluate in vitro pathogenicity of Trichoderma sp. isolates obtained from Urabá (Antioquia) on larvae of Ae. aegypti, and to determine the mean lethal concentration and mean lethal time of the most pathogenic isolate. Materials and methods. Using the sentinel larvae method of Ae. aegypti, fungi were isolated from water bodies in the Urabá region (Antioquia). The isolates were characterized morphologically and molecularly to determine their taxonomic identity. Pathogenicity tests were performed in vitro on Ae. aegypti larvae in the L2/L3 stages. Subsequently, a strain was selected to establish its mean lethal concentration and mean lethal time. Results. Trichoderma sp. strain AP-91 caused high mortality in larval populations of Aedes aegypti. We estimated a mean lethal concentration of 1.8 × 107 conidia/ml and a mean lethal time of 20.67 hours. Conclusion. The strain AP-91 showed potential for its use as biological control of Ae. aegypti, making it a suitable candidate for scale-up cultures applied to integrated vector management. This research suggests exploring compounds and enzymes produced by the AP-91 strain to understand better its pathogenicity. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label: Abstract (Spanish)
  Group: Ab
  Data: Introducción. Aedes aegypti es un vector importante de enfermedades arbovirales como el dengue, entre otras. Las estrategias tradicionales de control, como el uso de insecticidas, han perdido eficacia debido a la aparición de resistencia en las poblaciones de mosquitos. El control biológico y el uso de hongos biocontroladores se presentan como alternativas viables y amigables con el medio ambiente. Objetivo. Evaluar la patogenicidad in vitro de aislamientos del género Trichoderma -obtenidos del Urabá antioqueño- sobre larvas de Ae. aegypti, y determinar la concentración letal media (CL50) y el tiempo letal medio (TL50) del más patógenZonómica. Se llevaron a cabo pruebas de patogenicidad in vitro utilizando larvas de Ae. aegypti en los estadios L2 y L3. Posteriormente, se seleccionó una cepa para determinar su concentración letal media y tiempo letal medio. Resultados. La cepa AP-91 de Trichoderma sp. causó porcentajes altos de mortalidad en poblaciones de larvas de Ae. aegypti. Se obtuvo una concentración letal media de 1,8 × 107 conidios/ml y un tiempo letal medio de 20,67 horas. Conclusión. La cepa AP-91 tiene potencial para el control biológico de Ae. aegypti y puede ser una candidata idónea para usar en el manejo integrado de vectores mediante su cultivo escalado. La investigación sugiere explorar los compuestos y enzimas producidos por esta cepa para comprender mejor su patogenicidad. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.7705/biomedica.7520
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        Text: Spanish
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        Type: general
      – SubjectFull: ARBOVIRUS diseases
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      – SubjectFull: BIOLOGICAL control of mosquitoes
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      – SubjectFull: DISEASE vectors
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      – SubjectFull: BODIES of water
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      – TitleFull: Evaluación in vitro de la patogenicidad de los hongos aislados en la región de Urabá (Antioquia, Colombia) contra larvas de Aedes aegypti.
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