MÉTODOS MOLECULARES PARA LA DETECCIÓN DE PATÓGENOS EN ALIMENTOS.
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| Title: | MÉTODOS MOLECULARES PARA LA DETECCIÓN DE PATÓGENOS EN ALIMENTOS. |
|---|---|
| Alternate Title: | MOLECULAR METHODS FOR THE DETECTION OF PATHOGENS IN FOODS. |
| Authors: | Jiménez Soto, Lianet1 lianetjimenezsoto@gmail.com, Puig Peña, Yamila2 |
| Source: | Revista CENIC Ciencias Biologicas. 2024, Vol. 55, p173-179. 7p. |
| Subjects: | MOLECULAR biology, POLYMERASE chain reaction, FOODBORNE diseases, FOOD chemistry, DETECTION of microorganisms |
| Abstract (English): | For the prevention and control of foodborne diseases, many countries have established within their legislation "zero tolerance" for certain food pathogens. Based on this, rapid and modern techniques are necessary for detection; molecular methods allow us to satisfy such characteristics. With the aim of describing the usefulness of molecular biology techniques in food analysis, a documentary review of different methods was carried out, searches were carried out in Google Scholar, Medscape, Scielo and the most up-to-date information was included. Among the most frequently used tests for the analysis of food samples are: polymerase chain reaction, real-time polymerase chain reaction, microarrays, biosensors, high-throughput nucleic acid sequencing (pyrosequencing), and electrophoresis pulsed field gel; these techniques vary depending on the type of sample being analyzed and the purpose of the research. Molecular diagnostic techniques represent a promising alternative in the food field, due to their speed, high sensitivity and efficiency for the early detection of pathogenic microorganisms. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): | Para la prevención y el control de las enfermedades transmitidas por alimentos, en muchos países tienen establecido dentro de su legislación "tolerancia cero" para determinados patógenos alimenticios, en función de esto, son necesarias técnicas rápidas y modernas para la detección, los métodos moleculares permiten satisfacer tales características. Con el objetivo de describir la utilidad de las técnicas de biología molecular en el análisis de alimentos, se realizó una revisión documental de diferentes métodos, se realizaron búsquedas en Google académico, Medscape, Scielo y se incluyó la información actualizada. Entre las pruebas empleadas con mayor frecuencia para el análisis de muestras de alimentos se encuentran: reacción en cadena de la polimerasa, reacción en cadena de la polimerasa en tiempo real, microarreglos, biosensores, secuenciación de ácidos nucleicos de alto rendimiento (pirosecuenciación) y electroforesis en gel de campo pulsado; dichas técnicas varían según el propósito de la investigación y el tipo de muestra que se analiza. Las técnicas de diagnóstico molecular representan una alternativa prometedora en el campo de análisis microbiológico de los alimentos, debido a su rapidez, elevada sensibilidad y eficiencia para la detección temprana de microorganismos patógenos. [ABSTRACT FROM AUTHOR] |
| Copyright of Revista CENIC Ciencias Biologicas is the property of Centro Nacional de Investigaciones Cientificas and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | MedicLatina |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: lth DbLabel: MedicLatina An: 185332498 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: MÉTODOS MOLECULARES PARA LA DETECCIÓN DE PATÓGENOS EN ALIMENTOS. – Name: TitleAlt Label: Alternate Title Group: TiAlt Data: MOLECULAR METHODS FOR THE DETECTION OF PATHOGENS IN FOODS. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Jiménez+Soto%2C+Lianet%22">Jiménez Soto, Lianet</searchLink><relatesTo>1</relatesTo><i> lianetjimenezsoto@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Puig+Peña%2C+Yamila%22">Puig Peña, Yamila</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Revista+CENIC+Ciencias+Biologicas%22">Revista CENIC Ciencias Biologicas</searchLink>. 2024, Vol. 55, p173-179. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22MOLECULAR+biology%22">MOLECULAR biology</searchLink><br /><searchLink fieldCode="DE" term="%22POLYMERASE+chain+reaction%22">POLYMERASE chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22FOODBORNE+diseases%22">FOODBORNE diseases</searchLink><br /><searchLink fieldCode="DE" term="%22FOOD+chemistry%22">FOOD chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22DETECTION+of+microorganisms%22">DETECTION of microorganisms</searchLink> – Name: Abstract Label: Abstract (English) Group: Ab Data: For the prevention and control of foodborne diseases, many countries have established within their legislation "zero tolerance" for certain food pathogens. Based on this, rapid and modern techniques are necessary for detection; molecular methods allow us to satisfy such characteristics. With the aim of describing the usefulness of molecular biology techniques in food analysis, a documentary review of different methods was carried out, searches were carried out in Google Scholar, Medscape, Scielo and the most up-to-date information was included. Among the most frequently used tests for the analysis of food samples are: polymerase chain reaction, real-time polymerase chain reaction, microarrays, biosensors, high-throughput nucleic acid sequencing (pyrosequencing), and electrophoresis pulsed field gel; these techniques vary depending on the type of sample being analyzed and the purpose of the research. Molecular diagnostic techniques represent a promising alternative in the food field, due to their speed, high sensitivity and efficiency for the early detection of pathogenic microorganisms. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Abstract (Spanish) Group: Ab Data: Para la prevención y el control de las enfermedades transmitidas por alimentos, en muchos países tienen establecido dentro de su legislación "tolerancia cero" para determinados patógenos alimenticios, en función de esto, son necesarias técnicas rápidas y modernas para la detección, los métodos moleculares permiten satisfacer tales características. Con el objetivo de describir la utilidad de las técnicas de biología molecular en el análisis de alimentos, se realizó una revisión documental de diferentes métodos, se realizaron búsquedas en Google académico, Medscape, Scielo y se incluyó la información actualizada. Entre las pruebas empleadas con mayor frecuencia para el análisis de muestras de alimentos se encuentran: reacción en cadena de la polimerasa, reacción en cadena de la polimerasa en tiempo real, microarreglos, biosensores, secuenciación de ácidos nucleicos de alto rendimiento (pirosecuenciación) y electroforesis en gel de campo pulsado; dichas técnicas varían según el propósito de la investigación y el tipo de muestra que se analiza. Las técnicas de diagnóstico molecular representan una alternativa prometedora en el campo de análisis microbiológico de los alimentos, debido a su rapidez, elevada sensibilidad y eficiencia para la detección temprana de microorganismos patógenos. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Revista CENIC Ciencias Biologicas is the property of Centro Nacional de Investigaciones Cientificas and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=lth&AN=185332498 |
| RecordInfo | BibRecord: BibEntity: Languages: – Code: spa Text: Spanish PhysicalDescription: Pagination: PageCount: 7 StartPage: 173 Subjects: – SubjectFull: MOLECULAR biology Type: general – SubjectFull: POLYMERASE chain reaction Type: general – SubjectFull: FOODBORNE diseases Type: general – SubjectFull: FOOD chemistry Type: general – SubjectFull: DETECTION of microorganisms Type: general Titles: – TitleFull: MÉTODOS MOLECULARES PARA LA DETECCIÓN DE PATÓGENOS EN ALIMENTOS. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Jiménez Soto, Lianet – PersonEntity: Name: NameFull: Puig Peña, Yamila IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 02535688 Numbering: – Type: volume Value: 55 Titles: – TitleFull: Revista CENIC Ciencias Biologicas Type: main |
| ResultId | 1 |
