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DIVERSIDAD GENÓMICA DE Escherichia coli UROPATÓGENA EN AISLADOS CLÍNICOS DE SEIS PAÍSES DE LATINOAMÉRICA, 2018-2023.

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Title: DIVERSIDAD GENÓMICA DE Escherichia coli UROPATÓGENA EN AISLADOS CLÍNICOS DE SEIS PAÍSES DE LATINOAMÉRICA, 2018-2023.
Alternate Title: GENOMIC DIVERSITY OF UROPATHOGENIC Escherichia coli IN CLINICAL ISOLATES FROM SIX LATIN AMERICAN COUNTRIES, 2018-2023.
Authors: Caballero, Francesca1, Martinez-Ventura, Anne1,2, Cuicapuza, Diego1,3, Fajardo-Loyola, Alex4, Gutierrez-Ajalcriña, Rosmery5, Soto-Pastrana, Javier6, Asmat-Marrufo, Percy7, Barco-Yaipen de Vera, Evelyn8, Meza-Fernandez, Henry9, Chambi-Quispe, Mario10, Pino-Dueñas, Jimena11, Laura-Rivas, Nicomedes12, Briones-Alejo, Alexander13, Diaz-Rengifo, Pilar14, Peralta-Siesquen, Carlos15, Salvatierra, Guillermo1, Tsukayama, Pablo1,16 pablo.tsukayama@upch.pe, Marcos-Carbajal, Pool1,17
Source: Revista Peruana de Medicina Experimental y Salud Pública. 2025, Vol. 42 Issue 2, p1-10. 10p.
Subjects: MOLECULAR cloning, GENOMES, MULTIDRUG resistance, DRUG resistance in microorganisms, ESCHERICHIA coli
Abstract (English): Objective. To genetically characterize clinical isolates of uropathogenic Escherichia coli (UPEC) from hospitals in Peru and contextualize them against 127 additional UPEC genomes reported in six Latin American countries between 2018 and 2023. Materials and methods. The genomes of 16 Peruvian UPEC isolates were sequenced, assembled and supplemented with 127 genomes available in the NCBI public database. Serotypes, sequence types (STs), antimicrobial resistance (AMR) genes, and resistance-associated mutations were identified. A phylogenetic analysis was also conducted in order to determine evolutionary relations and distribution in phylogroups. Results. The ST131 clone was the most prevalent (42.7%), followed by ST1193 (13.3%). Phylogroup B2 was widely predominant (83.2%), with serotype O25:H4 standing out. The resistance genes blaTEM-1, blaCTX-M-15, and blaCTX-M-27 were identified with high frequency, as well as mutations in gyrA and parC associated with fluoroquinolone resistance, especially in the ST131 clone. Conclusion. Our findings show high circulation of high-risk UPEC clones, such as ST131 and ST1193, in Latin America, along with a notable burden of genes and mutations linked to multidrug resistance, highlighting the need to strengthen regional genomic surveillance. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Objetivo. Caracterizar genéticamente aislados clínicos de Escherichia coli uropatógena (UPEC) provenientes de hospitales en Perú y contextualizarlos frente a 127 genomas adicionales de UPEC reportados en seis países de Latinoamérica entre 2018 y 2023. Materiales y métodos. Se secuenciaron y ensamblaron los genomas de 16 aislados peruanos de UPEC y se complementaron con 127 genomas disponibles en la base de datos pública del NCBI. Se identificaron serotipos, secuenciotipos (ST), genes de resistencia antimicrobiana (RAM) y mutaciones asociadas a resistencia. Asimismo, se realizó un análisis filogenético para determinar relaciones evolutivas y distribución en filogrupos. Resultados. El clon ST131 fue el más prevalente (42,7%), seguido de ST1193 (13,3%). El filogrupo B2 predominó ampliamente (83.2%), destacando el serotipo O25:H4. Se identificaron con alta frecuencia los genes de resistencia blaTEM-1, blaCTX-M-15 y blaCTX-M-27, así como mutaciones en gyrA y parC asociadas a resistencia a fluoroquinolonas, especialmente en el clon ST131. Conclusión. Los hallazgos evidencian una alta circulación de clones de UPEC de alto riesgo, como ST131 y ST1193, en Latinoamérica, junto con una notable carga de genes y mutaciones vinculadas a resistencia a múltiples antimicrobianos, lo que resalta la necesidad de fortalecer la vigilancia genómica regional. [ABSTRACT FROM AUTHOR]
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Database: MedicLatina
Description
Abstract:Objective. To genetically characterize clinical isolates of uropathogenic Escherichia coli (UPEC) from hospitals in Peru and contextualize them against 127 additional UPEC genomes reported in six Latin American countries between 2018 and 2023. Materials and methods. The genomes of 16 Peruvian UPEC isolates were sequenced, assembled and supplemented with 127 genomes available in the NCBI public database. Serotypes, sequence types (STs), antimicrobial resistance (AMR) genes, and resistance-associated mutations were identified. A phylogenetic analysis was also conducted in order to determine evolutionary relations and distribution in phylogroups. Results. The ST131 clone was the most prevalent (42.7%), followed by ST1193 (13.3%). Phylogroup B2 was widely predominant (83.2%), with serotype O25:H4 standing out. The resistance genes blaTEM-1, blaCTX-M-15, and blaCTX-M-27 were identified with high frequency, as well as mutations in gyrA and parC associated with fluoroquinolone resistance, especially in the ST131 clone. Conclusion. Our findings show high circulation of high-risk UPEC clones, such as ST131 and ST1193, in Latin America, along with a notable burden of genes and mutations linked to multidrug resistance, highlighting the need to strengthen regional genomic surveillance. [ABSTRACT FROM AUTHOR]
ISSN:17264634
DOI:10.17843/rpmesp.2025.422.14299