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Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición.

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Title: Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición.
Alternate Title: Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement.
Authors: Enciso-Benavides, Javier1 javier.enciso.benavides@gmail.com, Garcia-Paita, Marlon1 marlon.garcia@unmsm.edu.pe, Alvarez-Vega, Santiago1 santiagoav1913@gmail.com, Tapia-Rojas, Salyoc1 stapiar@cientifica.edu.pe, Mayanga-Herrera, Ana1 amayanga@cientifica.edu.pe, Pons, Maria J.2 ma.pons.cas@gmail.com
Source: Revista del Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo. Oct-Dec2025, Vol. 18 Issue 4, p1-12. 12p.
Subjects: Cellular immunity, T cells, Immunoassay, Flow cytometry, Vaccination, Cytokines, Molecular diagnosis, SARS-CoV-2
Abstract (English): Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory Tcell-related response, either after exposure to the virus or vaccination against SARS-CoV2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Comprender la respuesta inmunitaria frente a la infección o vacunación contra SARSCoV-2 se ha convertido en un objetivo clave para entender la enfermedad, su progresión, y protección frente a futuras infecciones o posibles nuevas variantes que puedan surgir del virus. El objetivo de la presente revisión es destacar la relevancia de la respuesta inmunológica celular y resumir las diferentes metodologías descritas para el estudio de esta respuesta mediada por células T, de memoria, tanto tras la exposición al virus como de la vacunación frente al SARS-CoV-2. Para ello, se incluyen los ensayos de proliferación celular (CFSE, 3H- timidina), métodos de tipificación celular (citometría de flujo), los ensayos inmunológicos basados en la medición de citocinas (ELISA, ELISPOT, Multiplexado en suspensión) y el análisis de genes relacionados con la inmunidad celular mediante técnicas moleculares (RT-qPCR o qPCR y secuenciación). [ABSTRACT FROM AUTHOR]
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Database: MedicLatina
Description
Abstract:Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory Tcell-related response, either after exposure to the virus or vaccination against SARS-CoV2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included [ABSTRACT FROM AUTHOR]
ISSN:22255109