Elaboración y evaluación de un antisuero para la detección inmunohistoquímica del virus del Zika en tejidos fijados en aldehídos.

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Bibliographic Details
Title:	Elaboración y evaluación de un antisuero para la detección inmunohistoquímica del virus del Zika en tejidos fijados en aldehídos.
Alternate Title:	Preparation and evaluation of antisera for immunohistochemical detection of Zika virus in aldehyde-fixed tissues.
Authors:	Sarmiento-Lacera, Ladys¹ lsarmiento@ins.gov.co, Santamaría, Gerardo¹, Gómez, Claudia Yadira¹, Rosales, Alicia¹, Díaz, Taylor¹, Álvarez, Diego², Corchuelo, Sheryll¹, Rivera, Jorge¹, Muñoz, Alejandra³, Pinzón, Luz Mila⁴, Rengifo, Aura Caterine¹, Rincón, Verónica del Pilar⁵, Torres-Fernández, Orlando¹
Source:	Biomédica: Revista del Instituto Nacional de Salud. sep2026, Vol. 46 Issue 3, p489-498. 10p.
Subjects:	Immune serums, Immunohistochemistry, Neurons, Cross reactions (Immunology), Diagnostic reagents & test kits, Pathogenesis, Tissue fixation (Histology), Zika virus
Abstract (English):	Introduction. The nervous system is one of the main targets of the Zika virus. It is essential to have biological reagents to use as diagnostic support and in pathogenesis research that requires aldehyde-fixed tissues. Objective. To determine the utility of two types of antiserums prepared for the detection of Zika virus antigens in cells and tissues fixed in aldehydes. Materials and methods. The antiserums were produced in two New Zealand rabbits from a viral isolate from the 2016 Zika outbreak in Colombia. Freund's adjuvant was used for boosting in one of the animals, while Montanide adjuvant was used in the other. Immunoreaction titrations were performed on Zika-positive cells and tissues. The streptavidin-alkaline phosphatase and avidin-biotin-peroxidase methods were used. They were evaluated on experimentally infected samples for Zika and other related and unrelated viruses. Performance was evaluated with samples from other techniques used in the laboratory including vibratome sections, high-resolution optical microscopy samples, and transmission electron microscopy. Results. There was immunoreactivity to Zika virus antigens with both antiserums, mainly in neurons of the cerebral cortex. Immunostaining was also demonstrated in cell culture samples and tissues from experimentally infected animals. There was cross immunoreactivity with dengue and yellow fever viruses but not with rabies virus. Conclusions. The utility of the two antiserums for the detection of Zika virus in samples of human origin and from experimental animals obtained by different histological techniques used for the investigation of Zika virus disease pathogenesis was demonstrated. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	Introducción. El sistema nervioso es uno de los blancos del virus del Zika. Es fundamental disponer de reactivos biológicos para apoyo diagnóstico e investigaciones sobre patogénesis que requieren tejidos fijados en aldehídos. Objetivo. Determinar la utilidad de dos antisueros elaborados para la detección de antígenos del virus del Zika en células y tejidos fijados en aldehídos. Materiales y métodos. Los antisueros se produjeron en conejos Nueva Zelanda a partir de un aislamiento viral del brote de Zika del 2016 en Colombia. En uno de los animales se empleó adyuvante de Freund para los refuerzos y, en el otro, el adyuvante Montanide. Se realizaron titulaciones por inmunorreacciones en células y tejidos positivos para el virus del Zika. Se emplearon los métodos de estreptavidina-fosfatasa alcalina y avidina-biotinaperoxidasa. Los antisueros se evaluaron en muestras infectadas experimentalmente para el virus del Zika y para otros virus relacionados y no relacionados; también, se evaluaron con muestras de otras técnicas utilizadas en el laboratorio, como cortes obtenidos con vibrátomo, muestras para microscopía óptica de alta resolución y microscopía electrónica de transmisión. Resultados. Hubo inmunorreactividad a los antígenos del virus del Zika con ambos antisueros, principalmente en las neuronas de la corteza cerebral. Se demostró inmunotinción en las muestras de cultivos celulares y en los tejidos de animales infectados experimentalmente. Hubo inmunorreactividad cruzada con los virus del dengue y de la fiebre amarilla, pero no con el virus de la rabia. Conclusiones. Se demostró la utilidad de los dos antisueros para la detección del virus del Zika en muestras de origen humano y de animales de experimentación, obtenidas mediante diferentes técnicas histológicas utilizadas para la investigación de la patogénesis de la enfermedad por el virus del Zika. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	Introduction. The nervous system is one of the main targets of the Zika virus. It is essential to have biological reagents to use as diagnostic support and in pathogenesis research that requires aldehyde-fixed tissues. Objective. To determine the utility of two types of antiserums prepared for the detection of Zika virus antigens in cells and tissues fixed in aldehydes. Materials and methods. The antiserums were produced in two New Zealand rabbits from a viral isolate from the 2016 Zika outbreak in Colombia. Freund's adjuvant was used for boosting in one of the animals, while Montanide adjuvant was used in the other. Immunoreaction titrations were performed on Zika-positive cells and tissues. The streptavidin-alkaline phosphatase and avidin-biotin-peroxidase methods were used. They were evaluated on experimentally infected samples for Zika and other related and unrelated viruses. Performance was evaluated with samples from other techniques used in the laboratory including vibratome sections, high-resolution optical microscopy samples, and transmission electron microscopy. Results. There was immunoreactivity to Zika virus antigens with both antiserums, mainly in neurons of the cerebral cortex. Immunostaining was also demonstrated in cell culture samples and tissues from experimentally infected animals. There was cross immunoreactivity with dengue and yellow fever viruses but not with rabies virus. Conclusions. The utility of the two antiserums for the detection of Zika virus in samples of human origin and from experimental animals obtained by different histological techniques used for the investigation of Zika virus disease pathogenesis was demonstrated. [ABSTRACT FROM AUTHOR]
ISSN:	01204157
DOI:	10.7705/biomedica.7857