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DISEÑO Y ESTANDARIZACIÓN DE UNA PRUEBA DE PCR PARA EL DIAGNÓSTICO DE LA BARTONELOSIS CAUSADA POR Bartonella bacilliformis.

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Title: DISEÑO Y ESTANDARIZACIÓN DE UNA PRUEBA DE PCR PARA EL DIAGNÓSTICO DE LA BARTONELOSIS CAUSADA POR Bartonella bacilliformis.
Authors: Padilla R., Carlos1 cpadilla@ins.gob.pe, Ventura E., Gladys2
Source: Revista Peruana de Medicina Experimental y Salud Pública. 2003, Vol. 20 Issue 1, p5-8. 4p.
Subjects: VERRUGA peruana, DNA, BLOOD, MALARIA, NUCLEIC acids, DIAGNOSIS
Abstract (English): Objective: To design a PCR test for the diagnosis of Bartonellosis produced by Bartonella bacilliformis. Material and methods: ialB locus was used to design ialBF and ialBR primers, and PCR conditions were standardized using genomic DNA from a referral strain. Furthermore, the test was preliminary assessed in 12 clinical isolates from three Peruvian endemic areas and 10 whole blood samples of bartonelosis confirmed cases. Results: This test detected DNA from B. bacilliformis strains from three Peruvian endemic places of bartonellosis: Ancash, Cuzco and Lima. Genomic DNA of B. hensenlae, B. vinsonii, and other pathogens were not detected. In addition, this test was positive for ten whole blood samples of patients with confirmed bartonellosis and it was negative for five whole blood samples of patients with P. falciparum malaria. Conclusions: This PCR test could be useful for diagnosing bartonellosis caused by B. bacilliformis. [ABSTRACT FROM AUTHOR]
Abstract (Spanish): Objetivo: Diseñar una prueba de PCR para el diagnóstico de la Bartonelosis causada por Bartonella bacilliformis. Materiales y métodos: Se usó la secuencia del locus de invasión ialB para diseñar los oligonucleótidos ialBF y ialBR, además del ADN genómico purificado de una cepa referencial de B. bacilliformis para estandarizar las condiciones de la prueba. Finalmente, la prueba fue preliminarmente evaluada con 12 cepas de B. bacilliormis aisladas en 3 áreas endémicas y 10 muestras de sangre total de pacientes con Bartonelosis confirmada. Resultados: La prueba detectó el ADN de aislamientos de B. bacilliformis de 3 áreas bartonelósicas endémicas del Perú: Ancash, Cuzco y Lima; mientras que no detectó el ADN de B. hensenlae, ni de B. vinsonii, ni de otras bacterias y parásitos. Además, esta prueba fue positiva para 10 muestras sanguíneas de pacientes con bartonelosis confirmada y negativa para 5 muestras de pacientes con malaria por P. falciparum. Conclusión: Esta prueba de PCR podría ser útil para el diagnóstico de la bartonelosis causada por B. bacilliformis. [ABSTRACT FROM AUTHOR]
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Database: MedicLatina
Description
Abstract:Objective: To design a PCR test for the diagnosis of Bartonellosis produced by Bartonella bacilliformis. Material and methods: ialB locus was used to design ialBF and ialBR primers, and PCR conditions were standardized using genomic DNA from a referral strain. Furthermore, the test was preliminary assessed in 12 clinical isolates from three Peruvian endemic areas and 10 whole blood samples of bartonelosis confirmed cases. Results: This test detected DNA from B. bacilliformis strains from three Peruvian endemic places of bartonellosis: Ancash, Cuzco and Lima. Genomic DNA of B. hensenlae, B. vinsonii, and other pathogens were not detected. In addition, this test was positive for ten whole blood samples of patients with confirmed bartonellosis and it was negative for five whole blood samples of patients with P. falciparum malaria. Conclusions: This PCR test could be useful for diagnosing bartonellosis caused by B. bacilliformis. [ABSTRACT FROM AUTHOR]
ISSN:17264634