Caracterización Preliminar del Desoxicolato de Sodio de los Proveedores Merck y Fluka.

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Bibliographic Details
Title:	Caracterización Preliminar del Desoxicolato de Sodio de los Proveedores Merck y Fluka.
Authors:	Rodríguez Cabrera, Masiel¹ m_rodriguezcabrera@yahoo.com, González Fernández, Pablo², Bayolo Guanche, Juan Vicente², Barrera Padrón, Zarai³
Source:	Revista CENIC Ciencias Quimicas. 2005 Supplement, Vol. 36, p1. 9p.
Subjects:	DETERGENTS, EXTRACTION (Chemistry), SOLUBILIZATION, SOLUBILITY, PROTEINS
Abstract (English):	The action of a detergent in extraction and solubilization stages for proteins is a complex process of a difficult optimization and very dependent on a trial and error process. The knowledge of physical and chemical properties of detergents can make the choice process for a detergent much easier. Although this knowledge does not evade the behavior of the detergent in the biological system on which it works, if it can help the specialist to lead better the interpretation of the results after each trial and error process tried. The sidium desoxicolate is the detergent used in the production process of antimeningococal vacine serotype B in one of the extraction and solubilization stages in proteins. The characterization was given by the determination of superficial tension, the critical miscell concentration (CMC), the hidrophyle -- lypophyle balance and the absorbance to wave length of 260, 280 and 400 nm as a purity criterion for the comparison among batches from the different suppliers. Among the results we obtained that for the suppliers of the Merkc brand, the critical miscell concentration from the processed batches was less than for Fluka. No influence in the age of the batches about the miscelar critical concentration was observed, or about the decrease of superficial tension. The highest values of the hidrophyle -- lypophyle coincide within the CMC zone, which reasserts that the CMC range, calculated is the concentration zone which guarantees the higher solubility of proteins. The presence of protein impurities was estimated for both supplier and nucleic traces. The Fluka batches shoved a higher purity and similarity depon them. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	La acción de un detergente en etapas de extracción y solubilización de proteínas resulta un proceso complejo, de difícil optimización y muy dependiente de un proceso de prueba y error. El conocimiento de las propiedades físico-químicas de los detergentes puede hacer el proceso de elección de un detergente mucho más fácil. Aunque este conocimiento no elude el comportamiento del detergente en el sistema biológico sobre el cuál trabajará, si puede ayudar al especialista a conducir mejor la interpretación de los resultados después de cada proceso de prueba y error ensayado. El desoxicolato de sodio es el detergente empleado en el proceso de producción de la vacuna antimeningocócica serotipo B en una de las etapas de extracción y solubilización de proteínas. La caracterización estuvo dada por la determinación de la tensión superficial, la concentración miscelar crítica (CMC), el balance hidrófilo-lipófilo y la Absorvancia a longitudes de onda de 260, 280 y 400 nm como criterio de pureza para la comparación entre lotes de los distintos proveedores. Entre los resultados obtuvimos que para el proveedor de la firma Merck la concentración miscelar crítica de los lotes procesados fue menor que para Fluka. No se observó influencia de la edad de los lotes sobre la concentración miscelar crítica ni sobre la disminución de la tensión superficial. Los mayores valores del balance hidrófilo-lipófilo coincidieron dentro de la zona de CMC, lo cual reafirma que el rango de CMC calculado es la zona de concentraciones que garantiza la mayor solubilidad de proteínas. Se estimó la presencia de impurezas proteicas para ambos proveedores y trazas de ácidos nucleicos. Los lotes de Fluka presentaron mayor pureza y similitud entre ellos. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	The action of a detergent in extraction and solubilization stages for proteins is a complex process of a difficult optimization and very dependent on a trial and error process. The knowledge of physical and chemical properties of detergents can make the choice process for a detergent much easier. Although this knowledge does not evade the behavior of the detergent in the biological system on which it works, if it can help the specialist to lead better the interpretation of the results after each trial and error process tried. The sidium desoxicolate is the detergent used in the production process of antimeningococal vacine serotype B in one of the extraction and solubilization stages in proteins. The characterization was given by the determination of superficial tension, the critical miscell concentration (CMC), the hidrophyle -- lypophyle balance and the absorbance to wave length of 260, 280 and 400 nm as a purity criterion for the comparison among batches from the different suppliers. Among the results we obtained that for the suppliers of the Merkc brand, the critical miscell concentration from the processed batches was less than for Fluka. No influence in the age of the batches about the miscelar critical concentration was observed, or about the decrease of superficial tension. The highest values of the hidrophyle -- lypophyle coincide within the CMC zone, which reasserts that the CMC range, calculated is the concentration zone which guarantees the higher solubility of proteins. The presence of protein impurities was estimated for both supplier and nucleic traces. The Fluka batches shoved a higher purity and similarity depon them. [ABSTRACT FROM AUTHOR]
ISSN:	10158553