Contenido de sulforafano (1-isotiocianato-4-(metilsulfinil)-butano) en vegetales crucíferos.

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Bibliographic Details
Title:	Contenido de sulforafano (1-isotiocianato-4-(metilsulfinil)-butano) en vegetales crucíferos.
Alternate Title:	Sulforaphane (1-isothiocyanato-4-(methylsulfinyl)-butane) content in cruciferous vegetables.
Authors:	Campas-Baypoli, Olga N.¹, Bueno-Solano, Carolina¹, Martínez-Ibarra, Diana M.¹, Camacho-Gil, Francisco¹, Villa-Lerma, Alma G.¹, Rodríguez-Núñez, Jesús R.¹, López-Cervantes, Jaime¹, Sánchez-Machado, Dalia I.¹
Source:	Archivos Latinoamericanos de Nutrición. mar2009, Vol. 59 Issue 1, p95-100. 6p. 3 Charts, 1 Graph.
Subjects:	ISOCYANATES, THIOCYANATES, COLE crops, BROCCOLI, CABBAGE, INFLORESCENCES, HIGH performance liquid chromatography
Abstract (English):	Sulforaphane is an isothiocyanate which has antimicrobial and anticarcinogenic properties, this compound is found in a wide variety of plants from genus Brassica oleracea, being the most important broccoli and cabbage. The objective of this research was to quantify sulforaphane in the edible parts of broccoli and cabbage leaves by highperformance liquid chromatography (HPLC). Sample preparation for the quantification of sulforaphane include the conversion of glucoraphanin to sulforaphane (45 ± 2 °C for 2.5 h), extracted with dichloromethane, purification of the extract in columns of solid phase extraction and detection by HPLC- UV. Sulforaphane concentration in broccoli is in the range of 214 µg/g DW (stems) to 499 µg/g DW (inflorescences). The purple cabbage (101.99 µg/g DW) has values greater than the green cabbage (7.58 µg/g DW). The inflorescences of broccoli and red cabbage leaves are rich in sulforaphane. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	El sulforafano es un isotiocianato con propiedades antimicrobianas y anticarcinogénicas, se encuentra en una amplia variedad de vegetales del género Brassica oleracea, considerándose las más importantes el brócoli y repollo. El objetivo de esta investigación fue cuantificar sulforafano en las partes comestibles de brócoli y en hojas de repollo por cromatografía líquida de alta resolución (HPLC). La preparación de la muestra para la cuantificación del sulforafano incluye la conversión de glucorafanina a sulforafano (45 ± 2°C durante 2,5 h), extracción con diclorometano, purificación del extracto en columnas de extracción de fase sólida, y detección por HPLC-UV. En brócoli la concentración de sulforafano está en el rango de 214 µg/g bs (tallos) a 499 µg/g bs (inflorescencias). El repollo morado (101,99 µg/g bs) presentó valores mayores de sulforafano que el repollo verde (7,58 µg/g bs). Las inflorescencias de brócoli y las hojas de repollo morado son ricos en sulforafano. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	Sulforaphane is an isothiocyanate which has antimicrobial and anticarcinogenic properties, this compound is found in a wide variety of plants from genus Brassica oleracea, being the most important broccoli and cabbage. The objective of this research was to quantify sulforaphane in the edible parts of broccoli and cabbage leaves by highperformance liquid chromatography (HPLC). Sample preparation for the quantification of sulforaphane include the conversion of glucoraphanin to sulforaphane (45 ± 2 °C for 2.5 h), extracted with dichloromethane, purification of the extract in columns of solid phase extraction and detection by HPLC- UV. Sulforaphane concentration in broccoli is in the range of 214 µg/g DW (stems) to 499 µg/g DW (inflorescences). The purple cabbage (101.99 µg/g DW) has values greater than the green cabbage (7.58 µg/g DW). The inflorescences of broccoli and red cabbage leaves are rich in sulforaphane. [ABSTRACT FROM AUTHOR]
ISSN:	00040622