DIFERENCIAS EN LA MORFOLOGIA DE LAS CELULAS PROSTATICAS EN LA CIRCULACION SANGUINEA (CPCS), CELULAS CANCEROSAS AISLADAS (CCAS) EN LA MEDULA OSEA Y CELULAS CANCEROSAS INFILTRANDO MICROGRUMOS EN LA MEDULA OSEA DE PACIENTES CON CANCER PROSTATICO, DETECTADAS UTILIZANDO METODOS DE INMUMOCITOQUIMICA.

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Title:	DIFERENCIAS EN LA MORFOLOGIA DE LAS CELULAS PROSTATICAS EN LA CIRCULACION SANGUINEA (CPCS), CELULAS CANCEROSAS AISLADAS (CCAS) EN LA MEDULA OSEA Y CELULAS CANCEROSAS INFILTRANDO MICROGRUMOS EN LA MEDULA OSEA DE PACIENTES CON CANCER PROSTATICO, DETECTADAS UTILIZANDO METODOS DE INMUMOCITOQUIMICA.
Alternate Title:	MORPHOLOGICAL DIFFERENCES BETWEEN CIRCULATING PROSTATE CELLS IN BLOOD AND BONE MARROW AND THOSE INFILTRATING BONE MARROW FRAGMENTS IN PATIENTS WITH PROSTATE CANCER, DETECTED USING IMMUNOCYTOCHEMICAL METHODS.
Authors:	Murray, Nigel R.^1,2,3, Badínez V., Leonardo⁴, Cárdenas S., Mercedes⁵
Source:	Revista Chilena de Tecnología Médica. 2009, Vol. 29 Issue 1, p1477-1483. 7p.
Subjects:	CELL morphology, BLOOD, BONE marrow, PROSTATE cancer, CENTRIFUGATION, IMMUNOCYTOCHEMISTRY, PROSTATE-specific antigen, BIOPSY
Abstract (English):	Describe and determine morphological differences between prostate cells found in blood and bone marrow in men with prostate cancer. Methods and Patients: After written informed consent, samples of blood and bone marrow were obtained from men with histological confirmed prostate cancer. Mononuclear cells were obtained by differential centrifugation and a touch-prep made from the biopsy sample. Prostate cells were identified using immunocytochemistry and defined as cells positive for anti-prostate specific antigen and negative for anti-CD45 (pan-leucocyte). Results: From the 71 patients studied, cells found in blood and bone marrow aspirates were round or oval, whereas the cells detected in microfragments of the biopsy showed the characters of a typical cancer cell, irregular in shape and size with long cytoplasmic processes. Conclusions: These morphological differences could be explained in that there are 2 populations of cells, those in blood or bone marrow aspirates with a round or oval appearance are in transit, circulating between the blood and other tissues such as bone marrow, and those found in microfragments which represent true micrometastasis. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	Objetivo: Describir y determinar diferencias en la morfología celular entre células prostáticas en la circulación sanguínea y muestras de la médula ósea en pacientes con cáncer prostático. Métodos y Pacientes: Después de un consentimiento informado por escrito, muestras de sangre venosa y médula ósea fueron extraídas de pacientes con cáncer prostático histológicamente confirmado. Células mononucleares fueron obtenidas por centrifugación diferencial y una impronta preparada de la biopsia de la médula ósea. Las células prostáticas fueron identificadas con inmunocitoquímica, definida como antígeno prostático específico positivo y CD45 (pan-leucocito) negativo. Resultados: De los 71 sujetos estudiados, las células obtenidas en sangre y aspiración de la médula ósea, fueron redondas u ovaladas, a diferencia de las células en los microgrumos o fragmentos que tuvieron las características típicas de las células malignas: irregulares, con diferencias en tamaño y con largos procesos citoplasmáticos. Conclusiones: Las diferencias en la morfología pueden ser explicadas debido a que habrían 2 tipos de células, unas circulando o en tránsito entre la sangre y otros tejidos -como médula ósea- con apariencia redonda u ovalada y las células en los microgrumos o fragmentos, que se han infiltrado la membrana endoostial y representan verdadera micrometástasis. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

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Abstract:	Describe and determine morphological differences between prostate cells found in blood and bone marrow in men with prostate cancer. Methods and Patients: After written informed consent, samples of blood and bone marrow were obtained from men with histological confirmed prostate cancer. Mononuclear cells were obtained by differential centrifugation and a touch-prep made from the biopsy sample. Prostate cells were identified using immunocytochemistry and defined as cells positive for anti-prostate specific antigen and negative for anti-CD45 (pan-leucocyte). Results: From the 71 patients studied, cells found in blood and bone marrow aspirates were round or oval, whereas the cells detected in microfragments of the biopsy showed the characters of a typical cancer cell, irregular in shape and size with long cytoplasmic processes. Conclusions: These morphological differences could be explained in that there are 2 populations of cells, those in blood or bone marrow aspirates with a round or oval appearance are in transit, circulating between the blood and other tissues such as bone marrow, and those found in microfragments which represent true micrometastasis. [ABSTRACT FROM AUTHOR]
ISSN:	07160135