APLICACIÓN DE LAS MICROONDAS EN LABORATORIOS DE ANATOMÍA PATOLÓGICA.

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Bibliographic Details
Title:	APLICACIÓN DE LAS MICROONDAS EN LABORATORIOS DE ANATOMÍA PATOLÓGICA.
Alternate Title:	MICROWAVE APPLICATION FOR LABORATORY OF PATHOLOGY.
Authors:	Kroff B., Paula
Source:	Revista Chilena de Tecnología Médica. 2011, Vol. 31 Issue 2, p1637-1644. 8p.
Subjects:	PATHOLOGICAL laboratories, MEDICAL protocols, EMBEDDINGS (Mathematics), TISSUE engineering, STIFFNESS (Engineering), MICROWAVES, STANDARDIZATION
Abstract (English):	This study sets out the criteria to standardize procedures in pathology laboratories, with domestic microwave oven. We reviewed histological processing protocols, develop new use of an oven of 800 watts, according to physical principles of microwave emission and interaction with tissue. New protocols include fixation, embedding, staining and histochemical techniques. Were used fresh and fixed samples and positive controls appendix, stomach, skin, liver, kidney and lung for the histochemical techniques, defining the variables that affect the standardization and comparing the proposed procedures with traditional methods. Both salines solution and formalin (10%) retain the structure, eliminating toxic vapors with the first. At inclusion, the results depend: sample size, water content, tissue stiffness and thickness. There is an increase in quality and intensity on stains and reactions with microwave. The results indicate a large decrease in the time of the proceedings, which would allow early diagnosis and implementation of early therapy. Having considered all the criteria necessary to standardize procedures, allows pathology laboratories employ domestic microwave ovens with results that support a better prognosis for patients and lower cost for the laboratory. [ABSTRACT FROM AUTHOR]
Abstract (Spanish):	El presente estudio expone los criterios para estandarizar procedimientos en laboratorios de Anatomía Patológica, con horno de microondas doméstico. Se revisaron protocolos de procesamiento histológico, desarrollando nuevos, utilizando un horno de 800 watts, según principios físicos de emisión de microondas e interacción con el tejido. Los nuevos protocolos abarcan fijación, inclusión, tinciones y técnicas histoquímicas. Fueron empleadas muestras frescas y fijadas, y controles positivos de apéndice, estómago, piel, hígado, riñón y pulmón para las técnicas histoquímicas, definiendo las variables que afectan la estandarización y comparando los procedimientos propuestos con métodos tradicionales. Tanto el suero fisiológico como la formalina (10%), conservan las estructuras, eliminándose los vapores tóxicos con el primero. En la inclusión, los resultados dependen de: tamaño de la muestra, contenido de agua, dureza del tejido y grosor. Existe un aumento de calidad e intensidad con horno de microondas en las tinciones y reacciones. Los resultados indican una gran disminución en el tiempo de los procedimientos, lo que permitiría diagnósticos oportunos y la realización de terapias tempranas. El tener en cuenta todos los criterios necesarios para estandarizar los procedimientos, permite que los laboratorios de Anatomía Patológica empleen hornos de microondas domésticos con resultados que apoyan un mejor pronóstico a los pacientes y un menor costo para el laboratorio. [ABSTRACT FROM AUTHOR]
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Database:	MedicLatina

Description
Abstract:	This study sets out the criteria to standardize procedures in pathology laboratories, with domestic microwave oven. We reviewed histological processing protocols, develop new use of an oven of 800 watts, according to physical principles of microwave emission and interaction with tissue. New protocols include fixation, embedding, staining and histochemical techniques. Were used fresh and fixed samples and positive controls appendix, stomach, skin, liver, kidney and lung for the histochemical techniques, defining the variables that affect the standardization and comparing the proposed procedures with traditional methods. Both salines solution and formalin (10%) retain the structure, eliminating toxic vapors with the first. At inclusion, the results depend: sample size, water content, tissue stiffness and thickness. There is an increase in quality and intensity on stains and reactions with microwave. The results indicate a large decrease in the time of the proceedings, which would allow early diagnosis and implementation of early therapy. Having considered all the criteria necessary to standardize procedures, allows pathology laboratories employ domestic microwave ovens with results that support a better prognosis for patients and lower cost for the laboratory. [ABSTRACT FROM AUTHOR]
ISSN:	07160135