Site-specific protein modification using immobilized sortase in batch and continuous-flow systems.

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Title:	Site-specific protein modification using immobilized sortase in batch and continuous-flow systems.
Authors:	Witte MD; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Wu T; 1] Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA. [2] Oncology Medicinal Chemistry, Janssen Research and Development, Beerse, Belgium., Guimaraes CP; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Theile CS; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Blom AE; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Ingram JR; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Li Z; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Kundrat L; Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA., Goldberg SD; Centyrex, Janssen Research and Development, LLC, Spring House, Pennsylvania, USA., Ploegh HL; 1] Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA. [2] Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Source:	Nature protocols [Nat Protoc] 2015 Mar; Vol. 10 (3), pp. 508-16. Date of Electronic Publication: 2015 Feb 26.
Publication Type:	Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Journal Info:	Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
Database:	MEDLINE Ultimate

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ISSN:	1750-2799
DOI:	10.1038/nprot.2015.026