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Identification of nsp1 gene as the target of SARS-CoV-2 real-time RT-PCR using nanopore whole-genome sequencing.

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Title: Identification of nsp1 gene as the target of SARS-CoV-2 real-time RT-PCR using nanopore whole-genome sequencing.
Authors: Chan WM; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Ip JD; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Chu AW; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Yip CC; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., Lo LS; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., Chan KH; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Ng AC; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Poon RW; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., To WK; Department of Pathology, Princess Margaret Hospital, Hong Kong, China., Tsang OT; Department of Medicine and Geriatrics, Princess Margaret Hospital, Hong Kong, China., Leung WS; Department of Medicine and Geriatrics, Princess Margaret Hospital, Hong Kong, China., Kwan MY; Department of Paediatrics and Adolescent Medicine, Princess Margaret Hospital, Hong Kong, China., Chua GT; Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Chung TW; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., Hung IF; Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Kok KH; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China., Cheng VC; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., Chan JF; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., Yuen KY; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.; Department of Microbiology, Queen Mary Hospital, Hong Kong, China., To KK; State Key Laboratory for Emerging Infectious Diseases, Department of Microbiology, Carol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.; Department of Microbiology, Queen Mary Hospital, Hong Kong, China.
Source: Journal of medical virology [J Med Virol] 2020 Nov; Vol. 92 (11), pp. 2725-2734. Date of Electronic Publication: 2020 Jun 19.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:1096-9071
DOI:10.1002/jmv.26140