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Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing.

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Title: Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing.
Authors: Gleitz HFE; Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Current address: Department of Developmental Biology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Oncode Institute, Erasmus MC, Rotterdam, 3015GD, the Netherlands., Snoeren IAM; Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Current address: Department of Developmental Biology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Oncode Institute, Erasmus MC, Rotterdam, 3015GD, the Netherlands., Fuchs SNR; Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Current address: Department of Developmental Biology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Oncode Institute, Erasmus MC, Rotterdam, 3015GD, the Netherlands., Leimkühler NB; Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Department of Hematology, Oncology, Hemostaseology, and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University, Aachen 52074, Germany., Schneider RK; Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Current address: Department of Developmental Biology, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Oncode Institute, Erasmus MC, Rotterdam, 3015GD, the Netherlands.; Department of Hematology, Oncology, Hemostaseology, and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University, Aachen 52074, Germany.; Institute for Biomedical Engineering, Department of Cell Biology, Rheinisch-Westfälische Technische Hochschule Aachen University, Aachen, Germany.
Source: STAR protocols [STAR Protoc] 2021 May 12; Vol. 2 (2), pp. 100538. Date of Electronic Publication: 2021 May 12 (Print Publication: 2021).
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc
Database: MEDLINE Ultimate
Description
ISSN:2666-1667
DOI:10.1016/j.xpro.2021.100538