Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification.

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Title: Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification.
Authors: Han X; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.; Isotope Research Lab, Sichuan Agricultural University, Ya'an 625014, China., Burger LL; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA., Garcia-Galiano D; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA., Moenter SM; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA., Myers MG; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, MI 48109, USA., Olson DP; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA., Elias CF; Department of Molecular & integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA.
Source: STAR protocols [STAR Protoc] 2021 Jun 09; Vol. 2 (2), pp. 100589. Date of Electronic Publication: 2021 Jun 09 (Print Publication: 2021).
Publication Type: Journal Article; Research Support, N.I.H., Extramural
Journal Info: Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc
Database: MEDLINE Ultimate
Description
ISSN:2666-1667
DOI:10.1016/j.xpro.2021.100589