Insect cell expression and purification of recombinant SARS-COV-2 spike proteins that demonstrate ACE2 binding.
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| Title: | Insect cell expression and purification of recombinant SARS-COV-2 spike proteins that demonstrate ACE2 binding. |
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| Authors: | Struble LR; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA., Smith AL; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA., Lutz WE; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA., Grubbs G; Division of Viral Products, Center for Biologics Evaluation and Research (CBER), FDA, Silver Spring, Maryland, USA., Sagar S; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA., Bayles KW; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA., Radhakrishnan P; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA.; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.; Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA.; Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, USA., Khurana S; Division of Viral Products, Center for Biologics Evaluation and Research (CBER), FDA, Silver Spring, Maryland, USA., El-Gamal D; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA.; Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA., Borgstahl GEO; Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Media Center, Omaha, Nebraska, USA.; Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska, USA.; Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska, USA. |
| Source: | Protein science : a publication of the Protein Society [Protein Sci] 2022 May; Vol. 31 (5), pp. e4300. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Journal Info: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9211750 Publication Model: Print Cited Medium: Internet ISSN: 1469-896X (Electronic) Linking ISSN: 09618368 NLM ISO Abbreviation: Protein Sci Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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| ISSN: | 1469-896X |
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| DOI: | 10.1002/pro.4300 |
