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Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies.

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Title: Optimization of BRET saturation assays for robust and sensitive cytosolic protein-protein interaction studies.
Authors: Besson B; Technology Development Platform, Institut Pasteur Korea, 16, Daewangpangyo-ro 712 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea.; Institut Pasteur, Unité Dynamique des Lyssavirus et Adaptation à l'Hôte, 28 rue du docteur Roux, 75015, Paris, France.; Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, rue du Docteur Roux, 75015, Paris, France., Eun H; Technology Development Platform, Institut Pasteur Korea, 16, Daewangpangyo-ro 712 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea., Kim S; Technology Development Platform, Institut Pasteur Korea, 16, Daewangpangyo-ro 712 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea., Windisch MP; Applied Molecular Virology, Institut Pasteur Korea, 16, Daewangpangyo-ro 712 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea., Bourhy H; Institut Pasteur, Unité Dynamique des Lyssavirus et Adaptation à l'Hôte, 28 rue du docteur Roux, 75015, Paris, France., Grailhe R; Technology Development Platform, Institut Pasteur Korea, 16, Daewangpangyo-ro 712 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea. regis.grailhe@ip-korea.org.
Source: Scientific reports [Sci Rep] 2022 Jun 15; Vol. 12 (1), pp. 9987. Date of Electronic Publication: 2022 Jun 15.
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:2045-2322
DOI:10.1038/s41598-022-12851-9