NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of Wnt-3a to endogenous Frizzled 7 in a colorectal cancer model.

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Title: NanoBiT- and NanoBiT/BRET-based assays allow the analysis of binding kinetics of Wnt-3a to endogenous Frizzled 7 in a colorectal cancer model.
Authors: Grätz L; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden., Sajkowska-Kozielewicz JJ; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.; Department of Organic and Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland., Wesslowski J; Institute of Biological and Chemical Systems-Functional Molecular Systems, Karlsruhe Institute of Technology, Karlsruhe, Germany., Kinsolving J; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden., Bridge LJ; Department of Computer Science and Creative Technologies, University of the West England, Bristol, UK., Petzold K; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden., Davidson G; Institute of Biological and Chemical Systems-Functional Molecular Systems, Karlsruhe Institute of Technology, Karlsruhe, Germany., Schulte G; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden., Kozielewicz P; Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
Source: British journal of pharmacology [Br J Pharmacol] 2024 Oct; Vol. 181 (20), pp. 3819-3835. Date of Electronic Publication: 2023 May 06.
Publication Type: Journal Article
Journal Info: Publisher: Wiley Country of Publication: England NLM ID: 7502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5381 (Electronic) Linking ISSN: 00071188 NLM ISO Abbreviation: Br J Pharmacol Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:1476-5381
DOI:10.1111/bph.16090