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A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens.

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Title: A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens.
Authors: Guna A; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA.; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA., Page KR; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA., Replogle JM; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, 94158, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA., Esantsi TK; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA., Wang ML; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA.; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA., Weissman JS; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA., Voorhees RM; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA. voorhees@caltech.edu.; Howard Hughes Medical Institute Freeman Hrabowski Scholar, California Institute of Technology, Pasadena, CA, 91125, USA. voorhees@caltech.edu.
Source: BMC genomics [BMC Genomics] 2023 Oct 30; Vol. 24 (1), pp. 651. Date of Electronic Publication: 2023 Oct 30.
Publication Type: Journal Article
Journal Info: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens.
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  Data: <searchLink fieldCode="AU" term="%22Guna+A%22">Guna A</searchLink>; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA.; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Page+KR%22">Page KR</searchLink>; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA.<br /><searchLink fieldCode="AU" term="%22Replogle+JM%22">Replogle JM</searchLink>; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA, 94158, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Esantsi+TK%22">Esantsi TK</searchLink>; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Wang+ML%22">Wang ML</searchLink>; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA.; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Weissman+JS%22">Weissman JS</searchLink>; Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.<br /><searchLink fieldCode="AU" term="%22Voorhees+RM%22">Voorhees RM</searchLink>; Division of Biology and Biological Engineering, California Institute of Technology, 1200 E. California Ave, Pasadena, CA, 91125, USA. voorhees@caltech.edu.; Howard Hughes Medical Institute Freeman Hrabowski Scholar, California Institute of Technology, Pasadena, CA, 91125, USA. voorhees@caltech.edu.
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