Crystallographic fragment screening and deep mutational scanning of Zika virus NS2B-NS3 protease enable development of resistance-resilient inhibitors.

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Title: Crystallographic fragment screening and deep mutational scanning of Zika virus NS2B-NS3 protease enable development of resistance-resilient inhibitors.
Authors: Ni X; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Richardson RB; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Godoy AS; São Carlos Institute of Physics, University of São Paulo, Av. João Dagnone, 1100 - Jardim Santa Angelina, São Carlos, 13563-120, Brazil., Ferla MP; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Kikawa C; Medical Scientist Training Program, University of Washington, Seattle, WA, USA.; Department of Genome Sciences, University of Washington, Seattle, WA, USA.; Division of Basic Sciences, Fred Hutch Cancer Center, Seattle, WA, USA., Scheen J; Open Molecular Software Foundation, Davis, CA 95618, USA., Hannon WW; Division of Basic Sciences, Computational Biology Program, and Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, Washington, USA.; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, Washington, USA., Capkin E; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom., Lahav N; The Wohl Drug Discovery Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science., Balcomb BH; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Marples PG; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Fairhead M; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Wang S; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Williams EP; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Tomlinson CWE; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Aschenbrenner JC; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Lithgo RM; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Winokan M; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Giroud C; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Chandran AV; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Walsh M; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Thompson W; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Bloom JD; Division of Basic Sciences, Fred Hutch Cancer Center, Seattle, WA, USA.; Howard Hughes Medical Institute, Seattle, Washington, USA., Barr H; The Wohl Drug Discovery Institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science., Kirkegaard K; Department of Genetics, Stanford University, Palo Alto, California, USA.; Department of Microbiology and Immunology, Stanford University, Palo Alto, California, USA., Koekemoer L; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom., Fearon D; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK., Evans MJ; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA., von Delft F; Centre for Medicines Discovery, NDM research Building, Roosevelt Dr, Headington, Oxford OX3 7FZ, United Kingdom.; Diamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom.; Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, UK.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2025 Feb 13. Date of Electronic Publication: 2025 Feb 13.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2692-8205
DOI:10.1101/2024.04.29.591502