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Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire.

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Title: Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire.
Authors: Eden T; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Schaffrath AZ; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Wesolowski J; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Stähler T; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Tode N; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Richter N; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Schäfer W; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Hambach J; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Hermans-Borgmeyer I; Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Woens J; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Le Gall CM; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands., Wendler S; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany., Linke-Winnebeck C; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany., Stobbe M; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany., Budnicki I; Genovac GmbH, Freiburg, Germany., Wanney A; Genovac GmbH, Freiburg, Germany., Heitz Y; Genovac GmbH, Freiburg, Germany., Schimmelpfennig L; Genovac GmbH, Freiburg, Germany., Schweitzer L; Genovac GmbH, Freiburg, Germany., Zimmer D; Genovac GmbH, Freiburg, Germany., Stahl E; Preclinics GmbH, Potsdam, Germany., Seyfried F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Gebhardt AJ; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Dieckow L; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Riecken K; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Fehse B; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Bannas P; Department of Radiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Magnus T; Department of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Verdoes M; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands., Figdor CG; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands., Hartlepp KF; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany., Schleer H; Genovac GmbH, Freiburg, Germany., Füner J; Preclinics GmbH, Potsdam, Germany., Tomas NM; III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Haag F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Rissiek B; Department of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Mann AM; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany., Menzel S; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.; Core Facility Nanobodies, University of Bonn, Bonn, Germany., Koch-Nolte F; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. nolte@uke.de.
Source: Nature communications [Nat Commun] 2024 Jun 03; Vol. 15 (1), pp. 4728. Date of Electronic Publication: 2024 Jun 03.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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  Data: Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire.
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  Data: <searchLink fieldCode="AU" term="%22Eden+T%22">Eden T</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Schaffrath+AZ%22">Schaffrath AZ</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Wesolowski+J%22">Wesolowski J</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Stähler+T%22">Stähler T</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Tode+N%22">Tode N</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Richter+N%22">Richter N</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Schäfer+W%22">Schäfer W</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Hambach+J%22">Hambach J</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Hermans-Borgmeyer+I%22">Hermans-Borgmeyer I</searchLink>; Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Woens+J%22">Woens J</searchLink>; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Le+Gall+CM%22">Le Gall CM</searchLink>; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Wendler+S%22">Wendler S</searchLink>; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany.<br /><searchLink fieldCode="AU" term="%22Linke-Winnebeck+C%22">Linke-Winnebeck C</searchLink>; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany.<br /><searchLink fieldCode="AU" term="%22Stobbe+M%22">Stobbe M</searchLink>; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany.<br /><searchLink fieldCode="AU" term="%22Budnicki+I%22">Budnicki I</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Wanney+A%22">Wanney A</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Heitz+Y%22">Heitz Y</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Schimmelpfennig+L%22">Schimmelpfennig L</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Schweitzer+L%22">Schweitzer L</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Zimmer+D%22">Zimmer D</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Stahl+E%22">Stahl E</searchLink>; Preclinics GmbH, Potsdam, Germany.<br /><searchLink fieldCode="AU" term="%22Seyfried+F%22">Seyfried F</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Gebhardt+AJ%22">Gebhardt AJ</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Dieckow+L%22">Dieckow L</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Riecken+K%22">Riecken K</searchLink>; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Fehse+B%22">Fehse B</searchLink>; Research Department Cell and Gene Therapy, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Bannas+P%22">Bannas P</searchLink>; Department of Radiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Magnus+T%22">Magnus T</searchLink>; Department of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Verdoes+M%22">Verdoes M</searchLink>; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Figdor+CG%22">Figdor CG</searchLink>; Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, The Netherlands.<br /><searchLink fieldCode="AU" term="%22Hartlepp+KF%22">Hartlepp KF</searchLink>; ChromoTek GmbH, Martinsried, Germany - A part of Proteintech Group, Martinsried, Germany.<br /><searchLink fieldCode="AU" term="%22Schleer+H%22">Schleer H</searchLink>; Genovac GmbH, Freiburg, Germany.<br /><searchLink fieldCode="AU" term="%22Füner+J%22">Füner J</searchLink>; Preclinics GmbH, Potsdam, Germany.<br /><searchLink fieldCode="AU" term="%22Tomas+NM%22">Tomas NM</searchLink>; III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Haag+F%22">Haag F</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Rissiek+B%22">Rissiek B</searchLink>; Department of Neurology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Mann+AM%22">Mann AM</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.<br /><searchLink fieldCode="AU" term="%22Menzel+S%22">Menzel S</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.; Core Facility Nanobodies, University of Bonn, Bonn, Germany.<br /><searchLink fieldCode="AU" term="%22Koch-Nolte+F%22">Koch-Nolte F</searchLink>; Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. nolte@uke.de.
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