Optimization of ultracentrifugation-based method to enhance the purity and proteomic profiling depth of plasma-derived extracellular vesicles and particles.

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Title: Optimization of ultracentrifugation-based method to enhance the purity and proteomic profiling depth of plasma-derived extracellular vesicles and particles.
Authors: Wan Z; Drukier Institute of Children's Health Weill Cornell Medicine New York New York USA.; Department of Pediatrics Weill Cornell Medicine New York New York USA., Gu J; Drukier Institute of Children's Health Weill Cornell Medicine New York New York USA.; Department of Pediatrics Weill Cornell Medicine New York New York USA., Balaji U; Drukier Institute of Children's Health Weill Cornell Medicine New York New York USA.; Department of Pediatrics Weill Cornell Medicine New York New York USA., Bojmar L; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Meyer Cancer Center Weill Cornell Medicine New York New York USA.; Department of Clinical and Experimental Medicine Linköping University Linköping Sweden., Molina H; Proteomics Resource Center The Rockefeller University New York New York USA., Heissel S; Proteomics Resource Center The Rockefeller University New York New York USA., Pagano AE; Proteomics Resource Center The Rockefeller University New York New York USA., Peralta C; Proteomics Resource Center The Rockefeller University New York New York USA., Shaashua L; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Meyer Cancer Center Weill Cornell Medicine New York New York USA., Ismailgeci D; Department of Medicine Memorial Sloan Kettering Cancer Center New York New York USA., Narozniak HK; Department of Medicine Memorial Sloan Kettering Cancer Center New York New York USA., Song Y; Hepatopancreatobiliary Service, Department of Surgery Memorial Sloan Kettering Cancer Center New York New York USA., Jarnagin WR; Hepatopancreatobiliary Service, Department of Surgery Memorial Sloan Kettering Cancer Center New York New York USA., Kelsen DP; Department of Medicine Memorial Sloan Kettering Cancer Center New York New York USA., Bromberg J; Department of Medicine Memorial Sloan Kettering Cancer Center New York New York USA.; Department of Medicine Weill Cornell Medicine New York New York USA., Pascual V; Drukier Institute of Children's Health Weill Cornell Medicine New York New York USA.; Department of Pediatrics Weill Cornell Medicine New York New York USA., Zhang H; Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Meyer Cancer Center Weill Cornell Medicine New York New York USA.
Source: Journal of extracellular biology [J Extracell Biol] 2024 Jul 23; Vol. 3 (7), pp. e167. Date of Electronic Publication: 2024 Jul 23 (Print Publication: 2024).
Publication Type: Journal Article
Journal Info: Publisher: Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles Country of Publication: United States NLM ID: 9918382980506676 Publication Model: eCollection Cited Medium: Internet ISSN: 2768-2811 (Electronic) Linking ISSN: 27682811 NLM ISO Abbreviation: J Extracell Biol Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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ISSN:2768-2811
DOI:10.1002/jex2.167