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Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs.

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Title: Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs.
Authors: Marquart KF; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.; Institute of Molecular Health Sciences, ETH Zürich, Zürich, Switzerland., Mathis N; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Mollaysa A; Department of Quantitative Biomedicine, University of Zurich, Zürich, Switzerland., Müller S; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Kissling L; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Rothgangl T; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Schmidheini L; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland.; Institute of Molecular Health Sciences, ETH Zürich, Zürich, Switzerland., Kulcsár PI; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Allam A; Department of Quantitative Biomedicine, University of Zurich, Zürich, Switzerland., Kaufmann MM; Institute for Transfusion Medicine and Gene Therapy, Medical Center, University of Freiburg, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany., Matsushita M; Institute of Molecular Health Sciences, ETH Zürich, Zürich, Switzerland., Haenggi T; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland., Cathomen T; Institute for Transfusion Medicine and Gene Therapy, Medical Center, University of Freiburg, Freiburg, Germany.; Center for Chronic Immunodeficiency, Faculty of Medicine, University of Freiburg, Freiburg, Germany., Kopf M; Institute of Molecular Health Sciences, ETH Zürich, Zürich, Switzerland., Krauthammer M; Department of Quantitative Biomedicine, University of Zurich, Zürich, Switzerland., Schwank G; Institute of Pharmacology and Toxicology, University of Zurich, Zürich, Switzerland. schwank@pharma.uzh.ch.
Source: Nature methods [Nat Methods] 2024 Nov; Vol. 21 (11), pp. 2084-2093. Date of Electronic Publication: 2024 Sep 23.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: United States NLM ID: 101215604 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1548-7105 (Electronic) Linking ISSN: 15487091 NLM ISO Abbreviation: Nat Methods Subsets: MEDLINE
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