Precise kilobase-scale genomic insertions in mammalian cells using PASTE.

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Title: Precise kilobase-scale genomic insertions in mammalian cells using PASTE.
Authors: Fell CW; Harvard Medical School, Harvard University, Boston, MA, USA.; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.; Gene and Cell Therapy Institute, Mass General Brigham, Cambridge, MA, USA.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA., Schmitt-Ulms C; Harvard Medical School, Harvard University, Boston, MA, USA.; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.; Gene and Cell Therapy Institute, Mass General Brigham, Cambridge, MA, USA.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA.; Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA., Tagliaferri DV; Harvard Medical School, Harvard University, Boston, MA, USA.; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.; Gene and Cell Therapy Institute, Mass General Brigham, Cambridge, MA, USA.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA., Gootenberg JS; Harvard Medical School, Harvard University, Boston, MA, USA. jgootenb@bidmc.harvard.edu.; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA. jgootenb@bidmc.harvard.edu.; Gene and Cell Therapy Institute, Mass General Brigham, Cambridge, MA, USA. jgootenb@bidmc.harvard.edu.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA. jgootenb@bidmc.harvard.edu.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. jgootenb@bidmc.harvard.edu., Abudayyeh OO; Harvard Medical School, Harvard University, Boston, MA, USA. omar@abudayyeh.science.; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA. omar@abudayyeh.science.; Gene and Cell Therapy Institute, Mass General Brigham, Cambridge, MA, USA. omar@abudayyeh.science.; Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA, USA. omar@abudayyeh.science.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. omar@abudayyeh.science.
Source: Nature protocols [Nat Protoc] 2025 Jun; Vol. 20 (6), pp. 1546-1583. Date of Electronic Publication: 2024 Dec 15.
Publication Type: Journal Article; Review; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101284307 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1750-2799 (Electronic) Linking ISSN: 17502799 NLM ISO Abbreviation: Nat Protoc Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:1750-2799
DOI:10.1038/s41596-024-01090-z