Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells.
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| Title: | Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells. |
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| Authors: | Schafer JM; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Muli CS; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Heikal RA; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA., Dyba MA; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Tarasov SG; Biophysics Resource, Center for Structural Biology, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA., Stratton MM; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA, 01003, USA., Strieter ER; Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA; Molecular and Cellular Biology Graduate Program, College of Natural Sciences, University of Massachusetts, Amherst, MA, 01003, USA., Walters KJ; Protein Processing Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA. Electronic address: kylie.walters@nih.gov. |
| Source: | Protein expression and purification [Protein Expr Purif] 2025 Apr; Vol. 228, pp. 106661. Date of Electronic Publication: 2025 Jan 09. |
| Publication Type: | Journal Article |
| Journal Info: | Publisher: Academic Press Country of Publication: United States NLM ID: 9101496 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0279 (Electronic) Linking ISSN: 10465928 NLM ISO Abbreviation: Protein Expr Purif Subsets: MEDLINE |
| Database: | MEDLINE Ultimate |
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