Multi-locus CRISPRi targeting with a single truncated guide RNA.

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Title: Multi-locus CRISPRi targeting with a single truncated guide RNA.
Authors: Moore MM; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wekhande S; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Issner R; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Collins A; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Cruz AJ; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Liu YV; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Javed N; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Casaní-Galdón S; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Buenrostro JD; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA., Epstein CB; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Mattei E; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Doench JG; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Bernstein BE; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Shoresh N; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Najm FJ; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. fadinajm@broadinstitute.org.
Source: Nature communications [Nat Commun] 2025 Feb 04; Vol. 16 (1), pp. 1357. Date of Electronic Publication: 2025 Feb 04.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Database: MEDLINE Ultimate
Description
ISSN:2041-1723
DOI:10.1038/s41467-025-56144-x