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Multi-locus CRISPRi targeting with a single truncated guide RNA.

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Title: Multi-locus CRISPRi targeting with a single truncated guide RNA.
Authors: Moore MM; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Wekhande S; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Issner R; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Collins A; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Cruz AJ; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Liu YV; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Javed N; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Casaní-Galdón S; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Buenrostro JD; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA., Epstein CB; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Mattei E; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Doench JG; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Bernstein BE; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA., Shoresh N; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA., Najm FJ; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. fadinajm@broadinstitute.org.
Source: Nature communications [Nat Commun] 2025 Feb 04; Vol. 16 (1), pp. 1357. Date of Electronic Publication: 2025 Feb 04.
Publication Type: Journal Article
Journal Info: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
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  Data: <searchLink fieldCode="AU" term="%22Moore+MM%22">Moore MM</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Wekhande+S%22">Wekhande S</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Issner+R%22">Issner R</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Collins+A%22">Collins A</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Cruz+AJ%22">Cruz AJ</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Liu+YV%22">Liu YV</searchLink>; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Javed+N%22">Javed N</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA.<br /><searchLink fieldCode="AU" term="%22Casaní-Galdón+S%22">Casaní-Galdón S</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA.<br /><searchLink fieldCode="AU" term="%22Buenrostro+JD%22">Buenrostro JD</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Epstein+CB%22">Epstein CB</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Mattei+E%22">Mattei E</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Doench+JG%22">Doench JG</searchLink>; Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Bernstein+BE%22">Bernstein BE</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.; Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.; Departments of Cell Biology and Pathology, Harvard Medical School, Boston, MA, USA.<br /><searchLink fieldCode="AU" term="%22Shoresh+N%22">Shoresh N</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.<br /><searchLink fieldCode="AU" term="%22Najm+FJ%22">Najm FJ</searchLink>; Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. fadinajm@broadinstitute.org.
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  Data: <searchLink fieldCode="JN" term="%22101528555%22">Nature communications</searchLink> [Nat Commun] 2025 Feb 04; Vol. 16 (1), pp. 1357. <i>Date of Electronic Publication: </i>2025 Feb 04.
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