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Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting.

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Title: Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting.
Authors: Houmam S; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104., Siodlak D; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104., Pham KD; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA., Salinas-Salinas C; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104., Ocañas SR; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Neuroscience Program, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA., Freeman WM; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Oklahoma City Veterans Affairs Medical Center, Oklahoma City, OK, 73104, USA., Rice HC; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Neuroscience Program, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.
Source: BioRxiv : the preprint server for biology [bioRxiv] 2025 Aug 12. Date of Electronic Publication: 2025 Aug 12.
Publication Type: Journal Article; Preprint
Journal Info: Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet ISSN: 2692-8205 (Electronic) Linking ISSN: 26928205 NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE
Database: MEDLINE Ultimate
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  Data: Protocol to isolate oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons from a single mouse brain using magnetic-activated cell sorting.
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  Data: <searchLink fieldCode="AU" term="%22Houmam+S%22">Houmam S</searchLink>; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.<br /><searchLink fieldCode="AU" term="%22Siodlak+D%22">Siodlak D</searchLink>; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.<br /><searchLink fieldCode="AU" term="%22Pham+KD%22">Pham KD</searchLink>; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.<br /><searchLink fieldCode="AU" term="%22Salinas-Salinas+C%22">Salinas-Salinas C</searchLink>; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.<br /><searchLink fieldCode="AU" term="%22Ocañas+SR%22">Ocañas SR</searchLink>; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Neuroscience Program, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.<br /><searchLink fieldCode="AU" term="%22Freeman+WM%22">Freeman WM</searchLink>; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Oklahoma City Veterans Affairs Medical Center, Oklahoma City, OK, 73104, USA.<br /><searchLink fieldCode="AU" term="%22Rice+HC%22">Rice HC</searchLink>; Aging & Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA.; Department of Biochemistry & Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.; Center for Geroscience and Healthy Brain Aging, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104.; Neuroscience Program, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.
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  Data: <searchLink fieldCode="JN" term="%22101680187%22">BioRxiv : the preprint server for biology</searchLink> [bioRxiv] 2025 Aug 12. <i>Date of Electronic Publication: </i>2025 Aug 12.
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        Value: 10.1101/2025.08.08.666877
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              Text: 2025 Aug 12
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