Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM.

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Title: Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM.
Authors: Castel J; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France., Botzanowski T; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Brooks I; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Frechard A; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Bey G; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Schroeter M; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Del Nero E; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Debaene F; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Ciesielski F; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Zeyer D; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Cianferani S; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France. sarah.cianferani@unistra.fr., Morales R; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France. rmorales@novalix.com.
Source: Scientific reports [Sci Rep] 2025 Aug 26; Vol. 15 (1), pp. 31517. Date of Electronic Publication: 2025 Aug 26.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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ISSN:2045-2322
DOI:10.1038/s41598-025-16529-w