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Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM.

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Title: Deciphering molecular determinants of GPBAR1-Gs protein interactions by HDX-MS and cryo-EM.
Authors: Castel J; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France., Botzanowski T; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Brooks I; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Frechard A; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Bey G; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Schroeter M; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Del Nero E; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Debaene F; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Ciesielski F; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Zeyer D; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France., Cianferani S; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France. sarah.cianferani@unistra.fr., Morales R; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France. rmorales@novalix.com.
Source: Scientific reports [Sci Rep] 2025 Aug 26; Vol. 15 (1), pp. 31517. Date of Electronic Publication: 2025 Aug 26.
Publication Type: Journal Article
Journal Info: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Database: MEDLINE Ultimate
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  Data: <searchLink fieldCode="AU" term="%22Castel+J%22">Castel J</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Botzanowski+T%22">Botzanowski T</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Brooks+I%22">Brooks I</searchLink>; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Frechard+A%22">Frechard A</searchLink>; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Bey+G%22">Bey G</searchLink>; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Schroeter+M%22">Schroeter M</searchLink>; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Del+Nero+E%22">Del Nero E</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Debaene+F%22">Debaene F</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Ciesielski+F%22">Ciesielski F</searchLink>; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Zeyer+D%22">Zeyer D</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.; Department of Structural Biology, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France.<br /><searchLink fieldCode="AU" term="%22Cianferani+S%22">Cianferani S</searchLink>; Laboratoire de Spectrométrie de Masse BioOrganique, IPHC UMR 7178, Infrastructure Nationale de Protéomique ProFI, Université de Strasbourg, CNRS, UAR2048 CNRS, 67087, Strasbourg, France. sarah.cianferani@unistra.fr.<br /><searchLink fieldCode="AU" term="%22Morales+R%22">Morales R</searchLink>; Department of Biophysics, Novalix, 16 Rue d'Ankara, 67000, Strasbourg, France. rmorales@novalix.com.
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